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The Pekin duck programmed death ligand-2: cDNA cloning, genomic structure, molecular characterization and expression analysis.
Mariah
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Programmed death-1 (PD-1), upon engagement by its ligands, programmed dying ligand-1 (PD-L1) and programmed dying ligand-2 (PD-L2), gives alerts that attenuate adaptive immune responses. Right here we describe the identification of the Pekin duck PD-L2 (duPD-L2) and its gene construction.
The duPD-L2 cDNA encodes a 321 amino acid protein that has an amino acid id of 76% and 35% with rooster and human PD-L2, respectively. Mapping of the duPD-L2 cDNA with duck genomic sequences revealed an exonic construction much like that of the human Pdcd1lg2 gene. Homology modelling of the duPD-L2 protein was suitable with the murine PD-L2 ectodomain construction.
Residues recognized to be essential for PD-1 receptor binding of murine PD-L2 had been principally conserved in duPD-L2 inside sheets A and G and partially conserved inside sheets C and F. DuPD-L2 mRNA was constitutively expressed in all tissues examined with highest expression ranges in lung, spleen, cloaca, bursa, cecal tonsil, duodenum and really low ranges of expression in muscle, kidney and mind.
Lipopolysaccharide remedy of adherent duck PBMC upregulated duPD-L2 mRNA expression. Our work reveals evolutionary conservation of the PD-L2 ectodomain construction and residues essential for PD-1 binding in vertebrates together with fish. The data supplied might be helpful for additional investigation of the position of duPD-L2 within the regulation of duck adaptive immunity and exploration of PD-1-targeted immunotherapies within the duck hepatitis B an infection mannequin.
Identification of a Novel 5-HT2 Receptor CDNA within the Ovary tissues of Black Tiger Shrimp (Paneaus Monodon).
The aim of this experimental research is to isolate a 5-HT receptor from Penaeus monodon. The mRNA had been remoted from ovary (stage III ) of the wild broad inventory then reserve transcribed to cDNA by utilizing Oligo (dT) primer and superscript III enzyme. The template was amplified by PCR approach, used Taq DNA polymerase and two degenerate primers 5-HT-TM2 and 5-HT-TM6, equivalent to the conserved amino acid sequences of invertebrate 5-HT receptors.
After cloning, checking optimistic PCR product, and sequencing evaluation revealed an opened body of 404 acid nucleotides, which was excessive id of acid amine coding, and coded for 133 acid amines of protein G. These consequence and the 5-HT-TM2 primer expressed in that opened body had been evident presentation of 5-HT2 receptor in P. monodon.
cDNA cloning and mRNA expression of cat and canine Cdkal1.
The cyclin-dependent kinase 5 regulatory subunit-associated protein 1-like 1 (CDKAL1) gene encodes methylthiotransferase, and the gene comprises danger variants for sort 2 diabetes in people. On this research, we carried out complementary DNA cloning for Cdkal1 within the cat and canine and characterised the tissue expression profiles of its messenger RNA. Cat and canine Cdkal1 complementary DNA encoded 576 and 578 amino acids, exhibiting very excessive sequence homology to mammalian CDKAL1 (>88.4%).
Actual-time polymerase chain response analyses revealed that Cdkal1 messenger RNA is very expressed in easy muscle and that tissue distribution of Cdkal1 is analogous in cats and canines. Genotyping evaluation of single-nucleotide polymorphism for cat Cdkal1 revealed that overweight cats had totally different tendencies from regular cats. These findings counsel that the cat and canine Cdkal1 gene is very conserved amongst mammals and that cat Cdkal1 could also be a candidate marker for genetic analysis of weight problems.

Template-assisted synthesis of adenine-mutagenized cDNA by a retroelement protein complicated.
Range-generating retroelements (DGRs) create unparalleled ranges of protein sequence variation by mutagenic retrohoming. Sequence data is transferred from an invariant template area (TR), by an RNA intermediate, to a protein-coding variable area.
Selective infidelity at adenines throughout switch is a trademark of DGRs from disparate micro organism, archaea, and microbial viruses. We recapitulated selective infidelity in vitro for the prototypical Bordetella bacteriophage DGR. A fancy of the DGR reverse transcriptase bRT and pentameric accent variability determinant (Avd) protein together with DGR RNA had been mandatory and adequate for synthesis of template-primed, covalently linked RNA-cDNA molecules, as noticed in vivo.
We recognized RNA-cDNA molecules to be branched and most plausibly linked by 2′-5′ phosphodiester bonds. Adenine-mutagenesis was intrinsic to the bRT-Avd complicated, which displayed unprecedented promiscuity whereas reverse transcribing adenines of both DGR or non-DGR RNA templates. In distinction, bRT-Avd processivity was strictly depending on the template, occurring just for the DGR RNA.
This restriction was primarily attributable to a noncoding phase downstream of TR, which particularly sure Avd and created a privileged website for processive polymerization. Restriction to DGR RNA might defend the host genome from injury. These outcomes outline the early steps in a novel pathway for enormous sequence diversification.
Giant-Scale Investigation of Soybean Gene Capabilities by Overexpressing a Full-Size Soybean cDNA Library in Arabidopsis.
Molecular breeding has turn out to be an essential strategy for crop enchancment, and a prerequisite for molecular breeding is elucidation of the capabilities of genetic loci or genes. Soybean is among the most essential meals and oil crops worldwide.
Nevertheless, because of the problem of genetic transformation in soybean, research of its practical genomics lag far behind these of different crops corresponding to rice, which severely impairs the progress of molecular enchancment in soybean.
Right here, we describe an efficient large-scale technique to analyze the capabilities of soybean genes through overexpression of a full-length soybean cDNA library in Arabidopsis. The overexpression vector pJL12 was modified to be used within the development of a normalized full-length cDNA library. The constructed cDNA library confirmed good high quality; repetitive clones represented roughly 4%, insertion fragments had been roughly 2.2 kb, and the full-length fee was roughly 98%.
This cDNA library was then overexpressed in Arabidopsis, and roughly 2000 transgenic strains had been preliminarily obtained. Phenotypic analyses of the optimistic T1 transgenic vegetation confirmed that greater than 5% of the T1 transgenic strains displayed irregular developmental phenotypes, and roughly 1% of the transgenic strains exhibited probably favorable traits.
We randomly amplified Four genes with apparent phenotypes (enlarged seeds, yellowish leaves, extra branches, and dense siliques) and repeated the transgenic analyses in Arabidopsis. Subsequent phenotypic commentary demonstrated that these phenotypes had been certainly because of the overexpression of soybean genes. We consider our technique represents an efficient large-scale strategy to analyze the capabilities of soybean genes and additional reveal genes favorable for molecular enchancment in soybean.
An RNA Binding Peptide Consisting of 4 Varieties of Amino Acid by in Vitro Choice Utilizing cDNA Show.
RNA-protein interactions have a central position within the dwelling world. On this article, we examined whether or not primitive peptides (30 residues) consisting of 4 sorts of amino acid (Gly, Ala, Asp, and Val) may work together with tRNA as a mannequin of primitive RNAs within the RNA world.
Mouse Lactation elevated protein 2 (LACE1) ELISA Kit |
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Human Lactation elevated protein 1, LACE1 ELISA KIT |
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ELI-42677h | Lifescience Market | 96 Tests | EUR 988.8 |
Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit |
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abx385075-96tests | Abbexa | 96 tests | EUR 1093.2 |
Lace1 ELISA Kit| Rat Lactation elevated protein 1 ELISA Kit |
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EF018892 | Lifescience Market | 96 Tests | EUR 826.8 |
Lace1 ELISA Kit| Mouse Lactation elevated protein 1 ELISA Kit |
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EF015336 | Lifescience Market | 96 Tests | EUR 826.8 |
Stomach Lysate (7 Day Old) |
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1415-7 | ProSci | 0.1 mg | EUR 229.2 |
Description: Stomach tissue lysate (7 Day Old) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Fetus Lysate (7 Day Fetus) |
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1451-F7 | ProSci | 0.1 mg | EUR 229.2 |
Description: Fetus lysate (7 Day Fetus) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Heart Lysate (7 day old mouse) |
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1401-7 | ProSci | 0.1 mg | EUR 229.2 |
Description: Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
cDNA - Human Tumor Cell Line: MCF 7 |
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C1255830 | Biochain | 40 reactions | EUR 385 |
cDNA - Plant Normal Tissue: cDNA - Plant: Corn |
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C1634330 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Rice |
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C1634360 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Wheat |
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C1634390 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Orange |
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C1634340 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Potato |
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C1634350 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis |
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C1634310 | Biochain | 40 reactions | EUR 674 |
cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean |
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C1634370 | Biochain | 40 reactions | EUR 674 |
Genorise® Canine IL-7 cDNA Clone, 10 ug |
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GR159002 | Genorise Scientific | 10 µg | EUR 153 |
Novo? cDNA Kit |
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M1165-100 | Biovision | each | EUR 424.8 |
Novo? cDNA Kit |
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M1165-25 | Biovision | each | EUR 320.4 |
Chicken (Normal/Adult) Universal (pool of tissue cDNA) |
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CHUC-565 | Alpha Diagnostics | 10 rxn | EUR 498 |
cDNA - Lupus: Lung |
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C1236152Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Lupus: Colon |
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C1236090Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Lupus: Heart |
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C1236122Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Lupus: Liver |
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C1236149Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Asthma: Lung |
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C1236152Ld-1 | Biochain | 40 reactions | EUR 899 |
cDNA - Lupus: Kidney |
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C1236142Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Lupus: Spleen |
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C1236246Lup | Biochain | 40 reactions | EUR 737 |
Evo? cDNA Supermix |
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M1168-100 | Biovision | each | EUR 457.2 |
Evo? cDNA Supermix |
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M1168-25 | Biovision | each | EUR 320.4 |
cDNA - Lupus: stomach |
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C1236248Lup | Biochain | 40 reactions | EUR 737 |
Novo? cDNA Supermix |
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M1169-100 | Biovision | each | EUR 529.2 |
Novo? cDNA Supermix |
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M1169-25 | Biovision | each | EUR 346.8 |
cDNA - Emphysema: Lung |
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C1236152Ld-3 | Biochain | 40 reactions | EUR 899 |
cDNA - Pneumonia: Lung |
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C1236152Ld-4 | Biochain | 40 reactions | EUR 899 |
cDNA - Lupus: Pancreas |
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C1236188Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Bronchitis: Lung |
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C1236152Ld-2 | Biochain | 40 reactions | EUR 899 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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20-abx09801620ulSystems | Abbexa |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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20-abx09802120ulSystems | Abbexa |
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cDNA - hypertension: Vein |
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C1236020Hd-2 | Biochain | 40 reactions | EUR 729 |
cDNA Synthesis SuperMix |
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20-abx09801420ulSystems | Abbexa |
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cDNA - hypertension: Heart |
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C1236122Hd-2 | Biochain | 40 reactions | EUR 729 |
Evo™ cDNA Kit |
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M1164-100 | Biovision | each | Ask for price |
Evo™ cDNA Kit |
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M1164-25 | Biovision | each | Ask for price |
cDNA - hypertension: Artery |
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C1236013Hd-2 | Biochain | 40 reactions | EUR 729 |
cDNA - hypertension: Kidney |
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C1236142Hd-2 | Biochain | 40 reactions | EUR 729 |
cDNA - Arteriosclerosis: Aorta |
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C1236012Hd-4 | Biochain | 40 reactions | EUR 899 |
cDNA - Arteriosclerosis: Artery |
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C1236013Hd-4 | Biochain | 40 reactions | EUR 899 |
Human eNOS cDNA probe |
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eNOS51-D-2 | Alpha Diagnostics | 2 ug | EUR 534 |
cDNA - Lupus: Spinal Cord |
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C1236234Lup | Biochain | 40 reactions | EUR 737 |
Tetro cDNA Synthesis Kit |
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BIO-65042 | Bioline | 30 Reactions | Ask for price |
Tetro cDNA Synthesis Kit |
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BIO-65043 | Bioline | 100 Reactions | Ask for price |
EVery cDNA Synthesis Kit |
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EVery200B-1 | SBI | 20 preps | EUR 307 |
cDNA - Liver Cirrhosis: Lung |
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C1236152Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Skin |
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C1236218Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Brain |
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C1236035Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Colon |
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C1236090Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Heart |
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C1236122Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Liver |
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C1236149Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Lupus: Small Intestine |
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C1236226Lup | Biochain | 40 reactions | EUR 737 |
circRNA cDNA Synthesis Kit |
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G627 | ABM | 25 rxn (20 ul/rxn) | EUR 370.8 |
cDNA Probe Diluent Solution |
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AR0063 | BosterBio | 5mL | EUR 127.2 |
cDNA - Liver Cirrhosis: Kidney |
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C1236142Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Spleen |
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C1236246Lcs | Biochain | 40 reactions | EUR 729 |
Novo? Transcriptome cDNA Kit |
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M1167-100 | Biovision | each | EUR 1142.4 |
Novo? Transcriptome cDNA Kit |
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M1167-25 | Biovision | each | EUR 529.2 |
SensiFAST cDNA Synthesis Kit |
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BIO-65053 | Bioline | 50 Reactions | Ask for price |
SensiFAST cDNA Synthesis Kit |
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BIO-65053/S | Bioline | Sample | Ask for price |
SensiFAST cDNA Synthesis Kit |
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BIO-65054 | Bioline | 250 Reactions | Ask for price |
cDNA - Alzheimer's Disease: Brain |
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C1236035Alz | Biochain | 40 reactions | EUR 729 |
cDNA - Parkinson's Disease: Brain |
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C1236035Par | Biochain | 40 reactions | EUR 729 |
cDNA - Pulmonary Embolism: Lung |
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C1236152Ld-5 | Biochain | 40 reactions | EUR 899 |
cDNA - Liver Cirrhosis: Pancreas |
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C1236188Lcs | Biochain | 40 reactions | EUR 729 |
Plant Tissue cDNA: Arabidopsis |
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PC34-310 | Alpha Diagnostics | 10 rxn | EUR 498 |
cDNA - Liver Cirrhosis: Esophagus |
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C1236106Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Liver Cirrhosis: Diaphragm |
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C1236169Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - Dementia: Brain: Hippocampus |
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C1236052Dem | Biochain | 40 reactions | EUR 886 |
Human lactate (lactate) ELISA Kit |
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YLA3911HU-48T | Shanghai YL Biotech | 48T | EUR 435 |
Human lactate (lactate) ELISA Kit |
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YLA3911HU-96T | Shanghai YL Biotech | 96T | EUR 562.5 |
OneScriptPlus cDNA Synthesis Kit |
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G235 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis Kit |
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G236 | ABM | 100 x 20 ul reactions | EUR 202.8 |
cDNA - Depression: Brain: Hippocampus |
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C1236052Dep | Biochain | 40 reactions | EUR 886 |
Evo? cDNA Kit (gDNA Removal) |
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M1166-100 | Biovision | each | EUR 457.2 |
cDNA - Lupus: Liver: Left Lobe |
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C1236150Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Rat Normal Tissue: Lung |
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C1434152 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Lung |
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C1734152 | Biochain | 40 reactions | EUR 385 |
cDNA - Lupus: Liver: Right Lobe |
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C1236151Lup | Biochain | 40 reactions | EUR 737 |
cDNA - Rat Normal Tissue: Brain |
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C1434035 | Biochain | 40 reactions | EUR 385 |
cDNA - Rat Normal Tissue: Colon |
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C1434090 | Biochain | 40 reactions | EUR 578 |
cDNA - Rat Normal Tissue: Heart |
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C1434122 | Biochain | 40 reactions | EUR 385 |
cDNA - Rat Normal Tissue: Liver |
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C1434149 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Brain |
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C1734035 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Cecum |
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C1734089 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Heart |
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C1734122 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Liver |
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C1734149 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Tumor Tissue: Bone |
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C1235023-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Lung |
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C1235152 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Tumor Tissue: Nose |
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C1235178-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Skin |
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C1235218-10 | Biochain | 10 reactions | EUR 274 |
Human Adult cDNA Tissue: Lung |
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HA-152 | Alpha Diagnostics | 10 rxn | EUR 498 |
Human Adult cDNA Tissue: Skin |
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HA-218 | Alpha Diagnostics | 10 rxn | EUR 498 |
OneScriptPlus cDNA Synthesis SuperMix |
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G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
Lactate ELISA Kit| General Lactate ELISA Kit |
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EF019605 | Lifescience Market | 96 Tests | EUR 826.8 |
cDNA - Rat Normal Tissue: Kidney |
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C1434142 | Biochain | 40 reactions | EUR 385 |
cDNA - Rat Normal Tissue: Rectum |
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C1434206 | Biochain | 40 reactions | EUR 578 |
cDNA - Rat Normal Tissue: Spleen |
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C1434246 | Biochain | 40 reactions | EUR 385 |
cDNA - Rat Normal Tissue: Testis |
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C1434260 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Kidney |
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C1734142 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Rectum |
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C1734206 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Testis |
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C1734260 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Lung |
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C1334152 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Tumor Tissue: Brain |
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C1235035-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Colon |
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C1235090 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Tumor Tissue: Liver |
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C1235149 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Tumor Tissue: Ovary |
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C1235183-10 | Biochain | 10 reactions | EUR 525 |
cDNA - Rat Normal Tissue: Adipose |
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C1434003 | Biochain | 40 reactions | EUR 578 |
cDNA - Rat Normal Tissue: Bladder |
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C1434010 | Biochain | 40 reactions | EUR 385 |
cDNA - Rat Normal Tissue: Stomach |
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C1434248 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Bladder |
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C1734010 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Trachea |
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C1734160 | Biochain | 40 reactions | EUR 385 |
cDNA - Dog Normal Tissue: Stomach |
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C1734248 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Brain |
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C1334035 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Colon |
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C1334090 | Biochain | 40 reactions | EUR 578 |
cDNA - Mouse Normal Tissue: Heart |
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C1334122 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Liver |
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C1334149 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Tumor Tissue: Breast |
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C1235086 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Tumor Tissue: Kidney |
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C1235142 | Biochain | 40 reactions | EUR 578 |
cDNA - Human Tumor Tissue: Rectum |
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C1235206-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Uterus |
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C1235274 | Biochain | 40 reactions | EUR 578 |
Human Adult cDNA Tissue: Testis |
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HA-260 | Alpha Diagnostics | 10 rxn | EUR 498 |
Human Tumor Tissue: Breast cDNA |
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HT05-090 | Alpha Diagnostics | 10 rxn | EUR 498 |
cDNA - Rat Normal Tissue: Placenta |
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C1434200 | Biochain | 40 reactions | EUR 578 |
cDNA - Dog Normal Tissue: Pancreas |
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C1734188 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Kidney |
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C1334142 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Rectum |
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C1334206 | Biochain | 40 reactions | EUR 578 |
cDNA - Mouse Normal Tissue: Spleen |
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C1334246 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Testis |
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C1334260 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Tumor Tissue: Adrenal |
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C1235004-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Bladder |
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C1235010-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Stomach |
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C1235248 | Biochain | 40 reactions | EUR 578 |
cDNA - Liver Cirrhosis: Lymph Node |
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C1236161Lcs | Biochain | 40 reactions | EUR 729 |
cDNA - hypertension: Interventricular Septum |
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C1236130Hd-2 | Biochain | 40 reactions | EUR 729 |
cDNA Synthesis SuperMix for qPCR |
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20-abx09801920ulSystems | Abbexa |
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cDNA - Dog Normal Tissue: Esophagus |
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C1734106 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Adipose |
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C1334003 | Biochain | 40 reactions | EUR 578 |
cDNA - Mouse Normal Tissue: Bladder |
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C1334010 | Biochain | 40 reactions | EUR 385 |
cDNA - Mouse Normal Tissue: Stomach |
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C1334248 | Biochain | 40 reactions | EUR 385 |
cDNA - Human Tumor Tissue: Lymphoma |
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C1235161-10 | Biochain | 10 reactions | EUR 274 |
cDNA - Human Tumor Tissue: Pancreas |
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C1235188-10 | Biochain | 10 reactions | EUR 274 |
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By in vitro number of binding peptides utilizing the cDNA show methodology, a attribute peptide was chosen from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. Curiously, the chosen peptide sure to a single-stranded area together with a loop construction of an RNA molecule with some sequence specificity.
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