DNA Research Center Ltd

PATL1 Antibody: A Crucial Tool for RNA Processing Research

• Mariah
• February 28, 2025
• 0

Introduction to PATL1

PATL1 (Protein Associated with Topoisomerase II Homolog 1) is an essential RNA-binding protein involved in post-transcriptional gene regulation. It plays a critical role in mRNA decay, translation repression, and P-body formation, making it a key protein in cellular RNA metabolism (NIH, NCBI). Researchers studying RNA stability, gene expression, and neurodegenerative diseases frequently target PATL1 as an investigative marker (PubMed).

What is the PATL1 Antibody?

The PATL1 Antibody is a highly specific reagent used to detect PATL1 in biological samples. It is widely utilized in Western blotting, immunoprecipitation (IP), immunohistochemistry (IHC), and RNA-binding protein assays to study RNA processing and cellular stress responses (NCI, CDC).

Applications in Molecular Biology Research

1. Western Blotting (WB) – Detection of PATL1 protein expression in cell lysates (NCBI Gene).
2. Immunohistochemistry (IHC) – Visualization of PATL1 localization in tissue samples (Human Protein Atlas).
3. Immunoprecipitation (IP) – Analysis of PATL1 interaction with other RNA-binding proteins (NIH RNA Biology Program).
4. RNA Immunoprecipitation (RIP) – Study of PATL1-associated mRNA targets (GenBank).
5. Fluorescence Microscopy – High-resolution imaging of PATL1 in cellular compartments (NIH Microscopy Resource).
6. ELISA (Enzyme-Linked Immunosorbent Assay) – Quantitative measurement of PATL1 protein levels in biological samples (NIH ELISA Protocols).
7. RNA-Seq Analysis – Investigation of PATL1-regulated transcriptomes (NCBI SRA).

PATL1 in Disease Research

• Neurodegenerative Diseases: PATL1 has been linked to RNA regulation defects in Alzheimer’s and Parkinson’s disease (National Institute on Aging).
• Cancer: PATL1 dysregulation has been observed in various cancer types, making it a potential therapeutic target (National Cancer Institute).
• Viral Infections: PATL1 is involved in viral RNA stability and host immune responses, affecting diseases such as HIV and influenza (NIH Virology Program).
• Autoimmune Disorders: Emerging studies suggest that PATL1 plays a role in immune response regulation, potentially impacting systemic lupus erythematosus (SLE) and rheumatoid arthritis (NIH Autoimmune Research).

Antibody Validation and Quality Control

The PATL1 Antibody undergoes extensive validation protocols, including knockdown/knockout assays, peptide blocking, and cross-reactivity testing to ensure specificity and reproducibility (FDA Antibody Validation).

Additionally, mass spectrometry and proteomics approaches confirm the specificity of PATL1 antibody binding (NCBI Proteomics Database).

Key Databases and References

• UniProtKB – Protein sequence and functional data for PATL1 (UniProt).
• KEGG Pathway Database – PATL1’s role in RNA metabolism and cell signaling (KEGG).
• NCBI GEO DataSets – Transcriptomic datasets related to PATL1 expression (GEO).
• Protein Data Bank (PDB) – 3D structural information on PATL1 (PDB).
• The Human Protein Atlas – Comprehensive immunohistochemical data on PATL1 (HPA).
• Reactome Database – Annotated biological pathways involving PATL1 (Reactome).
• RNAcentral – Compilation of non-coding RNA interactions with PATL1 (RNAcentral).

Conclusion

The PATL1 Antibody is a powerful tool for RNA biology, translational regulation, and disease research. Its validated specificity makes it ideal for molecular and cellular biology applications.

By utilizing PATL1 Antibody in advanced experimental techniques, researchers can uncover novel insights into RNA metabolism, post-transcriptional gene regulation, and disease mechanisms.

For more information on validated antibodies, refer to The Antibody Registry (Antibody Registry) or consult NIH reagent resources (NIH Reagents).