Anti-AKAP13 Antibody: A Vital Tool for Signal Transduction Research
Introduction to AKAP13
AKAP13 (A-Kinase Anchoring Protein 13) is a crucial scaffolding protein involved in signal transduction, G-protein signaling, and cytoskeletal regulation. It plays a significant role in the localization of protein kinase A (PKA) and facilitates its interaction with Rho family GTPases, impacting cellular communication and cytoskeletal dynamics (NIH, NCBI). Due to its importance in cardiovascular function, cancer progression, and immune response, AKAP13 is widely studied in molecular biology and disease research (PubMed).
What is the Anti-AKAP13 Antibody?
The Anti-AKAP13 Antibody is a high-specificity reagent designed to detect AKAP13 in biological samples. It is commonly used in Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and fluorescence microscopy to study intracellular signaling pathways and cellular architecture (NCI, CDC).
Applications in Molecular Biology Research
- Western Blotting (WB) – Detection of AKAP13 protein expression in various cell types (NCBI Gene).
- Immunohistochemistry (IHC) – Visualization of AKAP13 localization in tissue sections (Human Protein Atlas).
- Immunoprecipitation (IP) – Identification of protein-protein interactions involving AKAP13 (NIH Protein Interaction Database).
- Fluorescence Microscopy – High-resolution imaging of AKAP13 dynamics in live and fixed cells (NIH Microscopy Resource).
- Gene Expression Studies – Analysis of AKAP13-related transcriptomic data using RNA sequencing (GEO).
AKAP13 in Disease Research
- Cancer: AKAP13 overexpression has been linked to breast and colorectal cancer, making it a potential therapeutic target (National Cancer Institute).
- Cardiovascular Diseases: AKAP13 is implicated in cardiac hypertrophy and heart failure, where its role in PKA signaling is critical (National Heart, Lung, and Blood Institute).
- Neurological Disorders: Dysregulated AKAP13 signaling has been associated with neurodegenerative diseases (National Institute on Aging).
- Immune System Regulation: AKAP13 participates in T-cell activation and cytokine signaling, impacting autoimmune conditions (NIH Autoimmune Research).
Antibody Validation and Quality Control
The Anti-AKAP13 Antibody undergoes rigorous validation, including peptide blocking assays, knockout/knockdown verification, and cross-reactivity testing, to ensure high specificity and reproducibility (FDA Antibody Validation). Additionally, mass spectrometry-based proteomics is used to confirm its binding accuracy (NCBI Proteomics Database).
Key Databases and References
- UniProtKB – Protein sequence and structural data for AKAP13 (UniProt).
- KEGG Pathway Database – AKAP13’s role in intracellular signaling and kinase anchoring (KEGG).
- NCBI GEO DataSets – Transcriptomic datasets related to AKAP13 (GEO).
- Protein Data Bank (PDB) – 3D structural insights into AKAP13 (PDB).
- Reactome Database – Annotated pathways involving AKAP13 (Reactome).
Conclusion
The Anti-AKAP13 Antibody is an essential tool for studying signal transduction, cytoskeletal organization, and disease mechanisms. With validated specificity, it is widely used in research areas such as cancer, cardiovascular diseases, and immunology.
For more information on validated antibodies, refer to The Antibody Registry (Antibody Registry) or explore NIH reagent resources (NIH Reagents).