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The Characterization of Immunoprotection Induced by a cDNA Clone Derived from the Attenuated Taiwan Porcine Epidemic Diarrhea Virus Pintung 52 Strain.
- Mariah
- 0
The porcine epidemic diarrhea virus (PEDV) poses a fantastic risk to the worldwide swine industries and the unreliable safety induced by the at the moment accessible vaccines stays a serious problem. We beforehand generated a genogroup 2b (G2b) PEDV Taiwan Pintung 52 (PEDVPT) pressure, PEDVPT-P96, and decided its promising host immune response in opposition to the virulent PEDVPT-P5 pressure.
To check the attenuation determinants of PEDVPT-P96 and set up a PEDVPT-P96-based recombinant vector as a vaccine platform for additional antigenicity modification, iPEDVPT-P96, a full-length cDNA clone of PEDVPT-P96, was established.
Evaluating to the parental PEDVPT-P96 virus, the iPEDVPT-P96 virus confirmed environment friendly replication kinetics with a delayed decline of viral load and related however rather more uniform plaque sizes in Vero cells. Within the 5-week-old piglet mannequin, fecal viral shedding was noticed within the PEDVPT-P96-inoculated piglets, whereas these inoculated with iPEDVPT-P96 confirmed neither detectable fecal viral shedding nor PEDV-associated medical indicators.
Furthermore, inoculation with iPEDVPT-P96 elicited comparable ranges of anti-PEDV particular plasma IgG and fecal/salivary IgA, neutralizing antibody titers, and related however much less efficient immunoprotection in opposition to the virulent PEDVPT-P5 problem in comparison with the parental PEDVPT-P96.
Within the current examine, an infectious cDNA clone of an attenuated G2b PEDV pressure was efficiently generated for the primary time, and the in vitro and in vivo information point out that iPEDVPT-P96 is additional attenuated however stays immunogenic in comparison with its parental PEDVPT-P96 viral inventory. The profitable growth of the iPEDVPT-P96 cDNA clone might enable for the manipulation of the viral genome to review viral pathogenesis and facilitate the fast growth of efficient vaccines.
‘Learn-through marking’ reveals differential nucleotide composition of read-through and truncated cDNAs in iCLIP.
We established a modified iCLIP protocol, known as ‘read-through marking’, which facilitates the detection of cDNAs that haven’t been truncated upon encountering the RNA-peptide complicated throughout reverse transcription (read-through cDNAs).
A big proportion of those cDNAs could be undesirable in an iCLIP library, because it might have an effect on the decision of the tactic. To this finish, we added an oligonucleotide to the 5′-end of RNA fragments-a 5′-marker-to mark the read-through cDNAs. By making use of this modified iCLIP protocol to PTBP1 and eIF4A3, we discovered that the beginning websites of read-through cDNAs are enriched in adenosines, whereas the remaining cDNAs have a markedly completely different sequence content material at their begins, preferentially containing thymidines.
This discovering in flip signifies that a lot of the reads in our iCLIP libraries are a product of truncation with invaluable info concerning the proteins’ RNA-binding websites. Thus, cDNA begin websites confidently determine a protein’s RNA-crosslink websites and we will account for the influence of read-through cDNAs by generally including a 5′-marker.
Simulation Examine of cDNA Dataset to Examine Doable Affiliation of Differentially Expressed Genes of Human THP1-Monocytic Cells in Most cancers Development Affected by Bacterial Shiga Toxins.
Shiga toxin (Stxs) is a household of structurally and functionally associated bacterial cytotoxins produced by Shigella dysenteriae serotype 1 and shigatoxigenic group of Escherichia coli that trigger shigellosis and hemorrhagic colitis, respectively.
Till just lately, it has been thought that Stxs solely inhibits the protein synthesis and induces expression to a restricted variety of genes in host cells, however current information confirmed that Stxs can set off a number of signaling pathways in mammalian cells and activate cell cycle and apoptosis.
To discover the modifications in gene expression induced by Stxs which have been proven in different methods to correlate with most cancers development, we carried out the simulated evaluation of cDNA dataset and located differentially expressed genes (DEGs) of human THP1-monocytic cells handled with Stxs.
On this examine, your complete information (handled and untreated replicates) was analyzed by statistical algorithms applied in Bioconductor packages. The output information was validated by the k-fold cross approach utilizing generalized linear Gaussian fashions.
A complete of 50 DEGs have been recognized. 7 genes together with TSLP, IL6, GBP1, CD274, TNFSF13B, OASL, and PNPLA3 have been significantly (<0.00005) associated to most cancers proliferation. The practical enrichment evaluation confirmed 6 down-regulated and 1 up-regulated genes.
Amongst these DEGs, IL6 was related to a number of cancers, particularly with leukemia, lymphoma, lungs, liver and breast cancers. The expected regulatory motifs of those genes embody conserved RELA, STATI, IRFI, NF-kappaB, PEND, HLF, REL, CEBPA, DI_2, and NFKB1 transcription issue binding websites (TFBS) concerned within the complicated organic capabilities. Thus, our findings counsel that Stxs has the potential as a invaluable device for higher understanding of therapy methods for a number of cancers.
cDNA Microarray Evaluation and Inflow Transporter OATP1B1 in Liver Cells After Publicity to Gadoxetate Disodium, a Gadolinium-based Distinction Agent in MRI Liver Imaging.
Gadoxetate disodium (Primovist or Eovist) is extensively used as a hepatospecific distinction agent throughout magnetic resonance imaging (MRI) examinations. Nevertheless, there is no such thing as a info figuring out whether or not gadoxetate disodium has a cytotoxic influence and/or impacts relative gene expression on liver cells. Within the present examine, we investigated the results of gadoxetate disodium on cytotoxicity and the degrees of gene expression in human regular Chang Liver cells.
The cytotoxic impact was detected through methyl thiazolyl tetrazolium (MTT) assay and 4′,6-diamidino-2-phenylindole (DAPI) staining. mRNA expression was monitored by cDNA microarray and quantitative PCR (qPCR) evaluation. The protein ranges have been decided by western blotting.
Gadoxetate disodium at 5 and 10 mM didn’t induce any cell cytotoxicity and morphological modifications in Chang Liver cells. Our information demonstrated that gadoxetate disodium considerably enhanced the expression of 29 genes and suppressed that of 27. The SLCO1C1 (solute provider natural anion transporter member of the family 1C1) mRNA expression was additionally elevated by 2.62-fold (p-value=0.0006) in gadoxetate disodium-treated cells.
Moreover, we additionally checked and located that gadoxetate disodium up-regulated natural anion transporter polypeptide 1B1 (OATP1B1) protein degree and elevated OATP uptake transporter gene SLCO1C1 mRNA expression.
Our outcomes present proof concerning that gadoxetate disodium may be no cytotoxic results on liver cells.
Manufacturing of a Beet chlorosis virus full-length cDNA clone by way of Gibson meeting and evaluation of organic properties.
Beet chlorosis virus (genus Polerovirus, household Luteoviridae), which is persistently transmitted by the aphid Myzus persicae, is a part of virus yellows in sugar beet and causes interveinal yellowing in addition to important yield loss in Beta vulgaris.
To permit reverse genetic research and exchange vector transmission, an infectious cDNA clone below cauliflower mosaic virus 35S management in a binary vector for agrobacterium-mediated an infection was constructed utilizing Gibson meeting. Following agroinoculation, the BChV full-length clone was in a position to induce a systemic an infection of the cultivated B. vulgaris.
Human Liver Cirrhosis Kidney Tissue Lysate |
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MBS8413965-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Paraffin Tissue Section - Liver Cirrhosis: Kidney |
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T2236142Lcs | Biochain | 5 slides | EUR 262 |
OCPB00551-100UG - Kidney Liver Cirrhosis Lysate |
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OCPB00551-100UG | Aviva Systems Biology | 0.1mg | EUR 739 |
Tissue, Total Protein, Human Disease, Liver Cirrhosis, Kidney |
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MBS657247-1mg | MyBiosource | 1mg | EUR 820 |
Tissue, Total Protein, Human Disease, Liver Cirrhosis, Kidney |
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MBS657247-5x1mg | MyBiosource | 5x1mg | EUR 3470 |
Genomic DNA - Liver Cirrhosis: Kidney, from a single donor |
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D1236142Lcs | Biochain | 50 ug | EUR 562 |
Tissue, Section, Human Disease, Liver Cirrhosis, Kidney (Paraffin) |
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MBS640354-5Sections | MyBiosource | 5Sections | EUR 510 |
Tissue, Section, Human Disease, Liver Cirrhosis, Kidney (Paraffin) |
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MBS640354-5x5Sections | MyBiosource | 5x5Sections | EUR 2145 |
cDNA - Liver Cirrhosis: Liver |
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C1236149Lcs | Biochain | 40 reactions | EUR 802 |
Tissue, Total RNA, Human Disease, Liver Cirrhosis, Kidney, BioGenomics |
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MBS638511-005mg | MyBiosource | 0.05mg | EUR 655 |
Tissue, Total RNA, Human Disease, Liver Cirrhosis, Kidney, BioGenomics |
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MBS638511-5x005mg | MyBiosource | 5x0.05mg | EUR 2735 |
Tissue, Genomic DNA, Human Disease, Liver Cirrhosis, Kidney, BioGenomics |
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MBS654423-005mg | MyBiosource | 0.05mg | EUR 715 |
Tissue, Genomic DNA, Human Disease, Liver Cirrhosis, Kidney, BioGenomics |
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MBS654423-5x005mg | MyBiosource | 5x0.05mg | EUR 3075 |
cDNA - Liver Cirrhosis: Lung |
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C1236152Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Skin |
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C1236218Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Brain |
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C1236035Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Colon |
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C1236090Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Heart |
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C1236122Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Spleen |
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C1236246Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Pancreas |
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C1236188Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Esophagus |
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C1236106Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Diaphragm |
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C1236169Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Lymph Node |
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C1236161Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Spinal Cord |
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C1236234Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Small Intestine |
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C1236226Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Small Intestine: Ileum |
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C1236227Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Small Intestine: Jejunum |
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C1236230Lcs | Biochain | 40 reactions | EUR 802 |
cDNA - Liver Cirrhosis: Small Intestine: Duodenum |
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C1236101Lcs | Biochain | 40 reactions | EUR 802 |
Liver Liver Cirrhosis Lysate |
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XBL-10358 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human liver tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Liver, BioGenomics |
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MBS652035-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Liver, BioGenomics |
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MBS652035-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Total RNA - Liver Cirrhosis: Liver |
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R1236149Lcs-50 | Biochain | 50 ug | EUR 460 |
Total Protein - Liver Cirrhosis: Liver |
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P1236149Lcs | Biochain | 1 mg | EUR 542 |
Liver Membrane Liver Cirrhosis Lysate |
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XBL-10671 | ProSci | 0.1 mg | EUR 752.1 |
Description: Human liver tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human liver tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated liver tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated liver tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Membrane Protein - Liver Cirrhosis: Liver |
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P3236149Lcs | Biochain | 0.1 mg | EUR 472 |
Lung Liver Cirrhosis Lysate |
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XBL-10363 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human lung tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Skin Liver Cirrhosis Lysate |
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XBL-10376 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human skin tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human skin tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skin tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skin tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Brain Liver Cirrhosis Lysate |
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XBL-10124 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human brain tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Colon Liver Cirrhosis Lysate |
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XBL-10334 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human colon tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Heart Liver Cirrhosis Lysate |
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XBL-10343 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human heart tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Ileum Liver Cirrhosis Lysate |
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XBL-10380 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human small intestine: Ileum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine: Ileum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine: Ileum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine: Ileum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Rectum Liver Cirrhosis Lysate |
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XBL-10375 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human rectum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human rectum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the rectum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The rectum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Spleen Liver Cirrhosis Lysate |
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XBL-10385 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human spleen tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human spleen tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spleen tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spleen tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Testis Liver Cirrhosis Lysate |
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XBL-10389 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human testis tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human testis tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the testis tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The testis tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Adrenal Liver Cirrhosis Lysate |
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XBL-10315 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human adrenal tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Jejunum Liver Cirrhosis Lysate |
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XBL-10382 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human small intestine: Jejunum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine: Jejunum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine: Jejunum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine: Jejunum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Thyroid Liver Cirrhosis Lysate |
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XBL-10390 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human thyroid tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human thyroid tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the thyroid tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The thyroid tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Skin, BioGenomics |
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MBS652450-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Skin, BioGenomics |
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MBS652450-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Lung, BioGenomics |
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MBS651928-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Lung, BioGenomics |
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MBS651928-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Human Liver Cirrhosis Liver Tissue Lysate |
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IHULRCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Liver Tissue Lysate |
Human Liver Cirrhosis Liver Tissue Lysate |
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MBS8414653-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Liver Tissue Lysate |
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MBS8414653-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Appendix Liver Cirrhosis Lysate |
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XBL-10317 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human appendix tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human appendix tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the appendix tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The appendix tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Duodenum Liver Cirrhosis Lysate |
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XBL-10337 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human duodenum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human duodenum tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the duodenum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The duodenum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Pancreas Liver Cirrhosis Lysate |
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XBL-10372 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human pancreas tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human pancreas tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the pancreas tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The pancreas tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Prostate Liver Cirrhosis Lysate |
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XBL-10374 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human prostate tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human prostate tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the prostate tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The prostate tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Esophagus Liver Cirrhosis Lysate |
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XBL-10339 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human esophagus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human esophagus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the esophagus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The esophagus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Diaphragm Liver Cirrhosis Lysate |
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XBL-10368 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human diaphragm tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human diaphragm tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the diaphragm tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The diaphragm tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Colon, BioGenomics |
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MBS652319-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Colon, BioGenomics |
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MBS652319-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Brain, BioGenomics |
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MBS652320-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Brain, BioGenomics |
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MBS652320-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Heart, BioGenomics |
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MBS652384-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Heart, BioGenomics |
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MBS652384-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Spleen, BioGenomics |
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MBS652239-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Spleen, BioGenomics |
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MBS652239-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Gallbladder Liver Cirrhosis Lysate |
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XBL-10340 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human gallbladder tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human gallbladder tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the gallbladder tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The gallbladder tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Total RNA - Liver Cirrhosis: Lung |
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R1236152Lcs-50 | Biochain | 50 ug | EUR 460 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Pancreas, BioGenomics |
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MBS652516-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Pancreas, BioGenomics |
|||
MBS652516-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Duodenum, BioGenomics |
|||
MBS651969-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Duodenum, BioGenomics |
|||
MBS651969-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Paraffin Tissue Section - Liver Cirrhosis: Liver |
|||
T2236149Lcs | Biochain | 5 slides | EUR 262 |
Total RNA - Liver Cirrhosis: Colon |
|||
R1236090Lcs-50 | Biochain | 50 ug | EUR 460 |
OCPB00553-100UG - Liver Liver Cirrhosis Lysate |
|||
OCPB00553-100UG | Aviva Systems Biology | 0.1mg | EUR 739 |
Total RNA - Liver Cirrhosis: Rectum |
|||
R1236206Lcs-50 | Biochain | 50 ug | EUR 460 |
Total RNA - Liver Cirrhosis: Spleen |
|||
R1236246Lcs-50 | Biochain | 50 ug | EUR 460 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Diaphragm, BioGenomics |
|||
MBS651903-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Diaphragm, BioGenomics |
|||
MBS651903-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Esophagus, BioGenomics |
|||
MBS652173-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Esophagus, BioGenomics |
|||
MBS652173-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Lymph node Liver Cirrhosis Lysate |
|||
XBL-10366 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human lymph node tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human lymph node tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lymph node tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lymph node tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Spinal cord Liver Cirrhosis Lysate |
|||
XBL-10125 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human spinal cord tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human spinal cord tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spinal cord tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spinal cord tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Total Protein - Liver Cirrhosis: Lung |
|||
P1236152Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Skin |
|||
P1236218Lcs | Biochain | 1 mg | EUR 542 |
Total RNA - Liver Cirrhosis: Pancreas |
|||
R1236188Lcs-50 | Biochain | 50 ug | EUR 460 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Lymph node, BioGenomics |
|||
MBS652040-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Lymph node, BioGenomics |
|||
MBS652040-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Spinal cord, BioGenomics |
|||
MBS652176-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Spinal cord, BioGenomics |
|||
MBS652176-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Total Protein - Liver Cirrhosis: Brain |
|||
P1236035Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Colon |
|||
P1236090Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Heart |
|||
P1236122Lcs | Biochain | 1 mg | EUR 542 |
Total RNA - Liver Cirrhosis: Diaphragm |
|||
R1236169Lcs-50 | Biochain | 50 ug | EUR 460 |
Total Protein - Liver Cirrhosis: Rectum |
|||
P1236206Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Spleen |
|||
P1236246Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Testis |
|||
P1236260Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Adrenal |
|||
P1236004Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Thyroid |
|||
P1236265Lcs | Biochain | 1 mg | EUR 542 |
Small Intestine Liver Cirrhosis Lysate |
|||
XBL-10377 | ProSci | 0.1 mg | EUR 796.2 |
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Total Protein - Liver Cirrhosis: Appendix |
|||
P1236006Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Pancreas |
|||
P1236188Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Prostate |
|||
P1236201Lcs | Biochain | 1 mg | EUR 542 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Small intestine, BioGenomics |
|||
MBS652026-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Small intestine, BioGenomics |
|||
MBS652026-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Total Protein - Liver Cirrhosis: Esophagus |
|||
P1236106Lcs | Biochain | 1 mg | EUR 542 |
Total Protein - Liver Cirrhosis: Diaphragm |
|||
P1236169Lcs | Biochain | 1 mg | EUR 542 |
Human Liver Cirrhosis Liver Lysate Membrane Fraction |
|||
IHULRCIRTLM100UG | Innovative research | each | EUR 1199 |
Description: Human Liver Cirrhosis Liver Lysate Membrane Fraction |
Human Liver Cirrhosis Liver Lysate Membrane Fraction |
|||
MBS8414654-01mg | MyBiosource | 0.1mg | EUR 1550 |
Human Liver Cirrhosis Liver Lysate Membrane Fraction |
|||
MBS8414654-5x01mg | MyBiosource | 5x0.1mg | EUR 6780 |
Membrane Protein - Liver Cirrhosis: Pancreas |
|||
P3236188Lcs | Biochain | 0.1 mg | EUR 472 |
Total Protein - Liver Cirrhosis: Gallbladder |
|||
P1236118Lcs | Biochain | 1 mg | EUR 542 |
OCPB00662-100UG - Liver Membrane Liver Cirrhosis Lysate |
|||
OCPB00662-100UG | Aviva Systems Biology | 0.1mg | EUR 689 |
Human Liver Cirrhosis Skin Tissue Lysate |
|||
IHUSKNCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Skin Tissue Lysate |
Human Liver Cirrhosis Lung Tissue Lysate |
|||
IHULGCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Lung Tissue Lysate |
Total Protein - Liver Cirrhosis: Lymph Node |
|||
P1236161Lcs | Biochain | 1 mg | EUR 542 |
Human Liver Cirrhosis Lung Tissue Lysate |
|||
MBS8414459-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Lung Tissue Lysate |
|||
MBS8414459-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Skin Tissue Lysate |
|||
MBS8419502-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Skin Tissue Lysate |
|||
MBS8419502-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Brain Tissue Lysate |
|||
IHUBRCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Brain Tissue Lysate |
Human Liver Cirrhosis Colon Tissue Lysate |
|||
IHUCLNCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Colon Tissue Lysate |
Human Liver Cirrhosis Heart Tissue Lysate |
|||
IHUHTCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Heart Tissue Lysate |
Human Liver Cirrhosis Ileum Tissue Lysate |
|||
IHUILMCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Ileum Tissue Lysate |
Total Protein - Liver Cirrhosis: Spinal Cord |
|||
P1236234Lcs | Biochain | 1 mg | EUR 542 |
Total RNA - Liver Cirrhosis: Small Intestine |
|||
R1236226Lcs-50 | Biochain | 50 ug | EUR 460 |
Human Liver Cirrhosis Heart Tissue Lysate |
|||
MBS8413247-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Heart Tissue Lysate |
|||
MBS8413247-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Ileum Tissue Lysate |
|||
MBS8413585-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Ileum Tissue Lysate |
|||
MBS8413585-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Brain Tissue Lysate |
|||
MBS138056-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Brain Tissue Lysate |
|||
MBS138056-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Colon Tissue Lysate |
|||
MBS139593-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Colon Tissue Lysate |
|||
MBS139593-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Tissue, Total Protein, Human Disease, Liver Cirrhosis, Liver |
|||
MBS657558-1mg | MyBiosource | 1mg | EUR 820 |
Tissue, Total Protein, Human Disease, Liver Cirrhosis, Liver |
|||
MBS657558-5x1mg | MyBiosource | 5x1mg | EUR 3470 |
Genomic DNA - Liver Cirrhosis: Liver, from a single donor |
|||
D1236149Lcs | Biochain | 50 ug | EUR 562 |
Human Liver Cirrhosis Testis Tissue Lysate |
|||
IHUTSTCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Testis Tissue Lysate |
Human Liver Cirrhosis Rectum Tissue Lysate |
|||
IHURCTCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Rectum Tissue Lysate |
Human Liver Cirrhosis Spleen Tissue Lysate |
|||
IHUSPCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Spleen Tissue Lysate |
Human Liver Cirrhosis Rectum Tissue Lysate |
|||
MBS8418431-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Rectum Tissue Lysate |
|||
MBS8418431-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Spleen Tissue Lysate |
|||
MBS8420014-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Spleen Tissue Lysate |
|||
MBS8420014-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Testis Tissue Lysate |
|||
MBS8420126-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Testis Tissue Lysate |
|||
MBS8420126-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Human Liver Cirrhosis Jejunum Tissue Lysate |
|||
IHUJEJCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Jejunum Tissue Lysate |
Human Liver Cirrhosis Jejunum Tissue Lysate |
|||
MBS8413807-01mg | MyBiosource | 0.1mg | EUR 1810 |
Human Liver Cirrhosis Jejunum Tissue Lysate |
|||
MBS8413807-5x01mg | MyBiosource | 5x0.1mg | EUR 7965 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Small intestine, Ileum, BioGenomics |
|||
MBS652396-40Tests | MyBiosource | 40Tests | EUR 995 |
Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Small intestine, Ileum, BioGenomics |
|||
MBS652396-5x40Tests | MyBiosource | 5x40Tests | EUR 4250 |
Human Liver Cirrhosis Duodenum Tissue Lysate |
|||
IHUDUOCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Duodenum Tissue Lysate |
Human Liver Cirrhosis Appendix Tissue Lysate |
|||
IHUAPXCIRTL100UG | Innovative research | each | EUR 1413 |
Description: Human Liver Cirrhosis Appendix Tissue Lysate |
×
The engineered virus was efficiently aphid-transmitted when acquired from contaminated B. vulgaris and displayed the identical host plant spectrum as wild-type virus. This new polerovirus infectious clone is a invaluable device to determine the viral determinants concerned in host vary and examine BChV protein perform, and can be utilized to display screen sugar beet for BChV resistance.
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