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Non-structural Proteins of Severe Fever With Thrombocytopenia Syndrome Virus Suppress RNA Synthesis in a Transcriptionally Active cDNA-Derived Viral RNA Synthesis System
Extreme fever with thrombocytopenia syndrome (SFTS) is an rising infectious illness attributable to the tick-borne SFTS bunyavirus (SFTSV) leading to a excessive fatality charge as much as 30%. SFTSV is a negative-strand RNA virus containing three single-stranded RNA genome segments designated as L, M, and S, which respectively, encode the RNA-dependent RNA polymerase (RdRp), glycoproteins Gn and Gc, and nucleoprotein (N) and non-structural proteins (NSs). NSs can type inclusion our bodies (IBs) in contaminated and transfected cells.
A earlier research has supplied a clue that SFTSV NSs could also be concerned in virus-like or viral RNA synthesis; nonetheless, the main points stay unclear. Our work described right here reveals that SFTSV NSs can downregulate virus-like RNA synthesis in a dose-dependent method inside a cDNA-derived viral RNA synthesis system, i.e., minigenome (-) and minigenome (+) methods primarily based on transfection, superinfection, and luciferase reporter exercise willpower; in the meantime, NSs additionally present a weak inhibitory impact on virus replication.
By utilizing co-immunoprecipitation (Co-IP) and RT-PCR mixed with site-directed mutagenesis, we discovered that NSs suppress virus-like RNA or virus replication by means of interacting with N however not with RdRp, and the detrimental regulatory impact correlates carefully with the IB construction it shaped however is just not related to its function of antagonizing host innate immune responses.
When the cytoplasmic construction of IB shaped by SFTSV NSs was disadvantaged, the inhibitory impact of NSs on virus-like RNA synthesis would weaken and even disappear.
Equally, we additionally evaluated different bandavirus NSs that can’t type IB in neither contaminated nor transfected cells, and the outcomes confirmed that the NSs of Heartland bandavirus (HRTV) didn’t present a big inhibitory impact on virus-like RNA synthesis inside a minigenome system. Our findings present experimental proof that SFTSV NSs take part in regulating virus-like or viral RNA synthesis and the detrimental impact could also be because of the NSs-N interplay.
Antimicrobial Peptides from Crops: A cDNA-Library Based mostly Isolation, Purification, Characterization Strategy and Elucidating Their Modes of Motion
Even in a pure ecosystem, vegetation are repeatedly threatened by numerous microbial ailments. To avoid wasting themselves from these numerous infections, vegetation construct a strong, multilayered immune system by means of their pure chemical compounds. Among the many a number of essential bioactive compounds possessed by vegetation’ immune methods, antimicrobial peptides (AMPs) rank within the first tier.
These AMPs are environmentally pleasant, anti-pathogenic, and don’t carry hurt to people. Antimicrobial peptides might be remoted in a number of methods, however recombinant protein manufacturing has change into more and more widespread in recent times, with the Escherichia coli expression system being probably the most extensively used.
Nevertheless, the efficacy of this expression system is compromised because of the problem of eradicating endotoxin from its system. Due to this fact, this evaluate suggests a high-throughput cDNA library-based plant-derived AMP isolation approach utilizing the Bacillus subtilis expression system. This technique might be carried out for large-scale screening of plant sources to categorise distinctive or homologous AMPs for the agronomic and utilized subject of plant research.
Moreover, this evaluate additionally focuses on the efficacy of plant AMPs, that are depending on their quite a few modes of motion and distinctive structural stability to operate in opposition to a variety of invaders. To conclude, the findings from this research will probably be helpful in investigating how novel AMPs are distributed amongst vegetation and supply detailed pointers for an efficient screening technique of AMPs.
Adapting 3′ Fast Amplification of CDNA Ends to Map Transcripts in Most cancers.
Maturation of eukaryotic mRNAs entails 3′ finish formation, which entails the addition of a poly(A) tail. With a purpose to map the three’ finish of a gene, the normal technique of alternative is 3′ speedy amplification of cDNA ends (3′ RACE). Protocols for 3′ RACE require the cautious design and number of nested primers throughout the 3′ untranslated area (3′ UTR) of the goal gene of curiosity.
Nevertheless, with a number of modifications the protocol can be utilized to incorporate your entire 3′ UTR and sequences throughout the open studying body (ORF), offering a extra complete image of the connection between the ORF and the three’ UTR.
That is along with identification of the polyadenylation sign (PAS), in addition to the cleavage and polyadenylation website supplied by standard 3′ RACE. Expanded 3′ RACE can detect uncommon 3′ UTRs, together with gene fusions throughout the 3′ UTR, and the sequence info can be utilized to foretell potential miRNA binding websites in addition to AU wealthy destabilizing components that will have an effect on the steadiness of the transcript.
Achieve of operate of sporadic/familial hemiplegic migraine-causing SCN1A mutations: Use of an optimized cDNA.
Introduction Familial hemiplegic migraine Three is an autosomal dominant headache dysfunction related to aura and transient hemiparesis, attributable to mutations of the neuronal voltage-gated sodium channel Nav1.1. Whereas a gain-of operate phenotype is mostly assumed to underlie familial hemiplegic migraine, this has not been totally explored. Certainly, a serious impediment in learning in vitro neuronal sodium channels is the problem in propagating and mutagenizing expression plasmids containing their cDNAs.
The intention of this work was to review the useful impact of two beforehand uncharacterized hemiplegic migraine inflicting mutations, Leu1670Trp (L1670W) and Phe1774Ser (F1774S). Strategies A novel SCN1A containing-plasmid was designed in silico and synthesized, and migraine mutations have been inserted on this background. Entire-cell patch clamp was carried out to research the useful properties of mutant Nav1.1 transiently expressed in Human Embryonic Kidney 293 cells.
Outcomes and conclusions We generated an optimized Nav1.1 expression plasmid that was very simple to deal with and used the novel plasmid to review the useful results of two migraine mutations. We noticed that L1670W, however not F1774S, decreased present density and that each mutations led to a dramatic enhance in persistent sodium currents, a depolarizing shift of the regular state-inactivation voltage-dependence, and a sooner restoration from inactivation.
The outcomes are per a serious gain-of operate impact underlying familial hemiplegic migraine 3. Our optimization technique will assist to characterize in an environment friendly method the impact in vitro of mutations of neuronal voltage-gated sodium channels.
Comparability of assorted RNA extraction strategies, cDNA preparation and isolation of calmodulin gene from a extremely melanized isolate of apple leaf blotch fungus Marssonina coronaria.
Marssonina coronaria causes apple blotch illness leading to extreme untimely defoliation, and is distributed in lots of main apple-growing areas on the earth. Efficient, dependable and high-quality RNA extraction is an indispensable process in any molecular biology research. No technique at present exists for RNA extraction from M. coronaria that produces a excessive amount of melanin-free RNA.
Due to this fact, we evaluated eight RNA extraction strategies together with handbook and business kits, to yield a adequate amount of high-quality and melanin-free RNA. Handbook strategies used right here resulted in low high quality and black coloured RNA pellets displaying the presence of melanin, regardless of all of the modifications employed to unique procedures.
Nevertheless, these strategies when coupled with clear up resulted in melanin-free RNA. Alternatively, all business kits used have been capable of yield high-quality melanin-free RNA having variable yields. TRIzol™ Reagent + RNA Clear & Concentrator™-5 and Ambion-PureLink® RNA Mini Package have been discovered to be the perfect strategies because the RNA extracted with these strategies from 15 day previous fungal tradition grown on strong medium have been freed from melanin with good yield.
Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat |
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| 4-20393 | CHI Scientific | ea | Ask for price |
Bovine SALIVARY PAROTID 25 EA* |
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| 57142-2 | Pel-Freez | 25 EA | EUR 493 |
Rabbit SALIVARY PAROTID Y 25 EA* |
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| 41241-2 | Pel-Freez | 25 EA | EUR 382.07 |
Rabbit SALIVARY PAROTID MATR 25 EA* |
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| 41342-2 | Pel-Freez | 25 EA | EUR 382.07 |
Frozen Tissue Section - Human Tumor: Parotid |
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| T1235190 | Biochain | 5 slides | EUR 405.6 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Anti-Parotid secretory protein (mouse) antibody |
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| STJ72465 | St John's Laboratory | 100 µg | EUR 312 |
cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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Novo? cDNA Kit |
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| M1165-100 | Biovision | EUR 424.8 | |
Novo? cDNA Kit |
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| M1165-25 | Biovision | EUR 320.4 | |
Evo? cDNA Supermix |
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| M1168-100 | Biovision | EUR 457.2 | |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | EUR 320.4 | |
Novo? cDNA Supermix |
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| M1169-100 | Biovision | EUR 529.2 | |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | EUR 346.8 | |
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
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| C1634310 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
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| C1634330 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
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| C1634340 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
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| C1634350 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
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| C1634360 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
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| C1634390 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Human anti-parotid duct antibody ELISA Kit |
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| 201-12-0451 | SunredBio | 96 tests | EUR 528 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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Rabbit anti parotid duct antibody ELISA kit |
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| E04A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit anti parotid duct antibody ELISA kit |
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| E04A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit anti parotid duct antibody ELISA kit |
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| E04A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Total Protein from Human Tumor Tissue: Parotid |
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| P1235190 | Biochain | 1 mg | EUR 553.2 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Pig anti parotid duct antibody ELISA kit |
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| E07A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig anti parotid duct antibody ELISA kit |
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| E07A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig anti parotid duct antibody ELISA kit |
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| E07A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti parotid duct antibody ELISA kit |
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| E01A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti parotid duct antibody ELISA kit |
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| E01A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti parotid duct antibody ELISA kit |
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| E01A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat anti parotid duct antibody ELISA kit |
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| E06A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat anti parotid duct antibody ELISA kit |
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| E06A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat anti parotid duct antibody ELISA kit |
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| E06A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse anti parotid duct antibody ELISA kit |
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| E03A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse anti parotid duct antibody ELISA kit |
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| E03A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse anti parotid duct antibody ELISA kit |
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| E03A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog anti parotid duct antibody ELISA kit |
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| E08A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog anti parotid duct antibody ELISA kit |
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| E08A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog anti parotid duct antibody ELISA kit |
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| E08A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti-parotid duct antibody ELISA Kit |
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| GA-E0467HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human anti-parotid duct antibody ELISA Kit |
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| GA-E0467HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Monkey anti parotid duct antibody ELISA kit |
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| E09A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey anti parotid duct antibody ELISA kit |
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| E09A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey anti parotid duct antibody ELISA kit |
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| E09A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Parotid secretory protein, Psp ELISA KIT |
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| ELI-16790m | Lifescience Market | 96 Tests | EUR 1038 |
Membrane Protein from Human Tumor Tissue: Parotid |
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| P3235190 | Biochain | 0.1 mg | EUR 759.6 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Paraffin Tissue Section - Human Adult Normal: Parotid |
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| T2234190 | Biochain | 5 slides | EUR 253.2 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Frozen Tissue Section - Human Adult Normal: Parotid |
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| T1234190 | Biochain | 5 slides | EUR 418.8 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Total RNA from Human Tumor Tissue: Parotid |
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| R1235190-10 | Biochain | 10 ug | EUR 421.2 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Human anti-parotid duct antibody ELISA Kit |
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| QY-E02562 | Qayee Biotechnology | 96T | EUR 433.2 |
First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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| 20-abx09801620ulSystems | Abbexa |
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First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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| 20-abx09802120ulSystems | Abbexa |
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cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
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| C1634370 | Biochain | 40 reactions | EUR 745.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Tetro cDNA Synthesis Kit |
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| BIO-65042 | Bioline | 30 Reactions | Ask for price |
Tetro cDNA Synthesis Kit |
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| BIO-65043 | Bioline | 100 Reactions | Ask for price |
SensiFAST cDNA Synthesis Kit |
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| BIO-65053 | Bioline | 50 Reactions | Ask for price |
SensiFAST cDNA Synthesis Kit |
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| BIO-65053/S | Bioline | Sample | Ask for price |
SensiFAST cDNA Synthesis Kit |
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| BIO-65054 | Bioline | 250 Reactions | Ask for price |
cDNA Probe Diluent Solution |
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| AR0063 | BosterBio | 5mL | EUR 127.2 |
cDNA from Arteriosclerosis: Aorta |
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| C1236012Hd-4 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from hypertension: Artery |
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| C1236013Hd-2 | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Arteriosclerosis: Artery |
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| C1236013Hd-4 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from hypertension: Vein |
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| C1236020Hd-2 | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Colon |
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| C1236090Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from hypertension: Heart |
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| C1236122Hd-2 | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Heart |
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| C1236122Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from hypertension: Kidney |
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| C1236142Hd-2 | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Kidney |
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| C1236142Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Liver |
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| C1236149Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Asthma: Lung |
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| C1236152Ld-1 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Bronchitis: Lung |
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| C1236152Ld-2 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Emphysema: Lung |
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| C1236152Ld-3 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Pneumonia: Lung |
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| C1236152Ld-4 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Lung |
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| C1236152Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Pancreas |
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| C1236188Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Spleen |
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| C1236246Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: stomach |
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| C1236248Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Human eNOS cDNA probe |
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| eNOS51-D-2 | Alpha Diagnostics | 2 ug | EUR 534 |
Evo™ cDNA Kit |
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| M1164-100 | Biovision | Ask for price | |
Evo™ cDNA Kit |
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| M1164-25 | Biovision | Ask for price | |
Novo? Transcriptome cDNA Kit |
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| M1167-100 | Biovision | EUR 1142.4 | |
Novo? Transcriptome cDNA Kit |
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| M1167-25 | Biovision | EUR 529.2 | |
OneScriptPlus cDNA Synthesis Kit |
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| G235 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis Kit |
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| G236 | ABM | 100 x 20 ul reactions | EUR 202.8 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G453 | ABM | 25 x 20 ul reactions | EUR 116.4 |
OneScriptPlus cDNA Synthesis SuperMix |
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| G454 | ABM | 100 x 20 ul reactions | EUR 202.8 |
circRNA cDNA Synthesis Kit |
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| G627 | ABM | 25 rxn (20 ul/rxn) | EUR 370.8 |
Plant Tissue cDNA: Arabidopsis |
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| PC34-310 | Alpha Diagnostics | 10 rxn | EUR 498 |
Pancreas Dissociation System 5 (Acinar, mouse parotid), Mouse |
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| 4-20375 | CHI Scientific | ea | Ask for price |
Pancreas Dissociation System 6 (Acinar, rat parotid), Rat |
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| 4-20376 | CHI Scientific | ea | Ask for price |
Parotid Dissociation System 1 (Epithelial), Mouse and Rat |
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| 4-20391 | CHI Scientific | ea | Ask for price |
Guinea pig anti parotid duct antibody ELISA kit |
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| E05A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig anti parotid duct antibody ELISA kit |
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| E05A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Guinea pig anti parotid duct antibody ELISA kit |
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| E05A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Total Protein from Human Adult Normal Tissue: Parotid |
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| P1234190 | Biochain | 1 mg | EUR 427.2 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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Paraffin Tissue Section - Human Parotid Tumor: Pleomorphic Adenoma |
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| T2235190-5 | Biochain | 5 slides | EUR 308.4 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Anti-parotid secretory protein (mouse aa146-160) antibody |
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| STJ72466 | St John's Laboratory | 100 µg | EUR 430.8 |
Total RNA from Human Adult Normal Tissue: Parotid |
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| R1234190-50 | Biochain | 50 ug | EUR 213.6 |
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Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA Synthesis SuperMix for qPCR |
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| 20-abx09801920ulSystems | Abbexa |
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cDNA from Alzheimer's Disease: Brain |
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| C1236035Alz | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Brain |
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| C1236035Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Parkinson's Disease: Brain |
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| C1236035Par | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Dementia: Brain: Hippocampus |
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| C1236052Dem | Biochain | 40 reactions | EUR 961.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Depression: Brain: Hippocampus |
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| C1236052Dep | Biochain | 40 reactions | EUR 961.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Colon |
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| C1236090Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Esophagus |
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| C1236106Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Heart |
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| C1236122Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from hypertension: Interventricular Septum |
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| C1236130Hd-2 | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Kidney |
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| C1236142Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Liver |
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| C1236149Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Lung |
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| C1236152Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Pulmonary Embolism: Lung |
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| C1236152Ld-5 | Biochain | 40 reactions | EUR 973.2 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Diaphragm |
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| C1236169Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Pancreas |
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| C1236188Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Skin |
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| C1236218Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Small Intestine |
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| C1236226Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Lupus: Spinal Cord |
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| C1236234Lup | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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cDNA from Liver Cirrhosis: Spleen |
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| C1236246Lcs | Biochain | 40 reactions | EUR 801.6 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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Monkey (Rhesus) cDNA Tissue: Thyroid |
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| MR34-265 | Alpha Diagnostics | 10 rxn | EUR 498 |
Evo? cDNA Kit (gDNA Removal) |
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| M1166-100 | Biovision | EUR 457.2 | |
Human Adult cDNA Tissue: Lung |
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| HA-152 | Alpha Diagnostics | 10 rxn | EUR 498 |
Human Adult cDNA Tissue: Skin |
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| HA-218 | Alpha Diagnostics | 10 rxn | EUR 498 |
Human Adult cDNA Tissue: Testis |
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| HA-260 | Alpha Diagnostics | 10 rxn | EUR 498 |
Human Tumor Tissue: Breast cDNA |
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| HT05-090 | Alpha Diagnostics | 10 rxn | EUR 498 |
Total-Transcriptome cDNA Synthesis Kit |
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| G904 | ABM | 25 reactions | EUR 268.8 |
Total-Transcriptome cDNA Synthesis Kit |
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| G905 | ABM | 100 reactions | EUR 652.8 |
Mouse iNOS (macrophage) cDNA probe |
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| iNOS61-D-2 | Alpha Diagnostics | 2 ug | EUR 534 |
Accuris qMax cDNA Synthesis Kit |
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| PR2100-C-100 | Benchmark Scientific | 1 PC | EUR 405.3 |
Accuris qMax cDNA Synthesis Kit |
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| PR2100-C-25 | Benchmark Scientific | 1 PC | EUR 170.83 |
Accuris qMax cDNA Synthesis Kit |
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| PR2100-C-250 | Benchmark Scientific | 1 PC | EUR 854.12 |
Accuris qMax cDNA Synthesis Kit |
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| PR2100-C-S | Benchmark Scientific | 1 PC | EUR 93.31 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-050 | GenDepot | 50 rxns | EUR 498 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-100 | GenDepot | 2X50 rxns | EUR 1016.4 |
amfiRivert cDNA Synthesis Master Mix |
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| R5101-200 | GenDepot | 4X50 rxns | EUR 1453.2 |
amfiRivet cDNA Synthesis 2X Buffer |
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| R5102-050 | GenDepot | 500ul | EUR 160.8 |
amfiRivet cDNA Synthesis 2X Buffer |
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| R5102-100 | GenDepot | 2x500ul | EUR 230.4 |
pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus] |
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| CD510VB-1 | SBI | >1 x 10^6 IFUs | EUR 943.2 |
RNA extracted by this improved methodology was utilized for RT-PCR, subsequent PCR amplification, and isolation of calmodulin gene sequences from M. coronaria and contaminated apple leaf items. These strategies are extra time efficient than conventional strategies and take solely an hour to finish. To our information, that is the primary report on the strategy of isolation of high-quality RNA for cDNA synthesis in addition to isolation of the calmodulin gene sequence from this fungus.
