Non-structural Proteins of Severe Fever With Thrombocytopenia Syndrome Virus Suppress RNA Synthesis in a Transcriptionally Active cDNA-Derived Viral RNA Synthesis System

Non-structural Proteins of Severe Fever With Thrombocytopenia Syndrome Virus Suppress RNA Synthesis in a Transcriptionally Active cDNA-Derived Viral RNA Synthesis System

Extreme fever with thrombocytopenia syndrome (SFTS) is an rising infectious illness attributable to the tick-borne SFTS bunyavirus (SFTSV) leading to a excessive fatality charge as much as 30%. SFTSV is a negative-strand RNA virus containing three single-stranded RNA genome segments designated as L, M, and S, which respectively, encode the RNA-dependent RNA polymerase (RdRp), glycoproteins Gn and Gc, and nucleoprotein (N) and non-structural proteins (NSs). NSs can type inclusion our bodies (IBs) in contaminated and transfected cells.
A earlier research has supplied a clue that SFTSV NSs could also be concerned in virus-like or viral RNA synthesis; nonetheless, the main points stay unclear. Our work described right here reveals that SFTSV NSs can downregulate virus-like RNA synthesis in a dose-dependent method inside a cDNA-derived viral RNA synthesis system, i.e., minigenome (-) and minigenome (+) methods primarily based on transfection, superinfection, and luciferase reporter exercise willpower; in the meantime, NSs additionally present a weak inhibitory impact on virus replication.
By utilizing co-immunoprecipitation (Co-IP) and RT-PCR mixed with site-directed mutagenesis, we discovered that NSs suppress virus-like RNA or virus replication by means of interacting with N however not with RdRp, and the detrimental regulatory impact correlates carefully with the IB construction it shaped however is just not related to its function of antagonizing host innate immune responses.
When the cytoplasmic construction of IB shaped by SFTSV NSs was disadvantaged, the inhibitory impact of NSs on virus-like RNA synthesis would weaken and even disappear.
Equally, we additionally evaluated different bandavirus NSs that can’t type IB in neither contaminated nor transfected cells, and the outcomes confirmed that the NSs of Heartland bandavirus (HRTV) didn’t present a big inhibitory impact on virus-like RNA synthesis inside a minigenome system. Our findings present experimental proof that SFTSV NSs take part in regulating virus-like or viral RNA synthesis and the detrimental impact could also be because of the NSs-N interplay.

Antimicrobial Peptides from Crops: A cDNA-Library Based mostly Isolation, Purification, Characterization Strategy and Elucidating Their Modes of Motion

Even in a pure ecosystem, vegetation are repeatedly threatened by numerous microbial ailments. To avoid wasting themselves from these numerous infections, vegetation construct a strong, multilayered immune system by means of their pure chemical compounds. Among the many a number of essential bioactive compounds possessed by vegetation’ immune methods, antimicrobial peptides (AMPs) rank within the first tier.
These AMPs are environmentally pleasant, anti-pathogenic, and don’t carry hurt to people. Antimicrobial peptides might be remoted in a number of methods, however recombinant protein manufacturing has change into more and more widespread in recent times, with the Escherichia coli expression system being probably the most extensively used.
Nevertheless, the efficacy of this expression system is compromised because of the problem of eradicating endotoxin from its system. Due to this fact, this evaluate suggests a high-throughput cDNA library-based plant-derived AMP isolation approach utilizing the Bacillus subtilis expression system. This technique might be carried out for large-scale screening of plant sources to categorise distinctive or homologous AMPs for the agronomic and utilized subject of plant research.
Moreover, this evaluate additionally focuses on the efficacy of plant AMPs, that are depending on their quite a few modes of motion and distinctive structural stability to operate in opposition to a variety of invaders. To conclude, the findings from this research will probably be helpful in investigating how novel AMPs are distributed amongst vegetation and supply detailed pointers for an efficient screening technique of AMPs.

Adapting 3′ Fast Amplification of CDNA Ends to Map Transcripts in Most cancers.

Maturation of eukaryotic mRNAs entails 3′ finish formation, which entails the addition of a poly(A) tail. With a purpose to map the three’ finish of a gene, the normal technique of alternative is 3′ speedy amplification of cDNA ends (3′ RACE). Protocols for 3′ RACE require the cautious design and number of nested primers throughout the 3′ untranslated area (3′ UTR) of the goal gene of curiosity.
Nevertheless, with a number of modifications the protocol can be utilized to incorporate your entire 3′ UTR and sequences throughout the open studying body (ORF), offering a extra complete image of the connection between the ORF and the three’ UTR.
That is along with identification of the polyadenylation sign (PAS), in addition to the cleavage and polyadenylation website supplied by standard 3′ RACE. Expanded 3′ RACE can detect uncommon 3′ UTRs, together with gene fusions throughout the 3′ UTR, and the sequence info can be utilized to foretell potential miRNA binding websites in addition to AU wealthy destabilizing components that will have an effect on the steadiness of the transcript.

Achieve of operate of sporadic/familial hemiplegic migraine-causing SCN1A mutations: Use of an optimized cDNA.

Introduction Familial hemiplegic migraine Three is an autosomal dominant headache dysfunction related to aura and transient hemiparesis, attributable to mutations of the neuronal voltage-gated sodium channel Nav1.1. Whereas a gain-of operate phenotype is mostly assumed to underlie familial hemiplegic migraine, this has not been totally explored. Certainly, a serious impediment in learning in vitro neuronal sodium channels is the problem in propagating and mutagenizing expression plasmids containing their cDNAs.
The intention of this work was to review the useful impact of two beforehand uncharacterized hemiplegic migraine inflicting mutations, Leu1670Trp (L1670W) and Phe1774Ser (F1774S). Strategies A novel SCN1A containing-plasmid was designed in silico and synthesized, and migraine mutations have been inserted on this background. Entire-cell patch clamp was carried out to research the useful properties of mutant Nav1.1 transiently expressed in Human Embryonic Kidney 293 cells.
Outcomes and conclusions We generated an optimized Nav1.1 expression plasmid that was very simple to deal with and used the novel plasmid to review the useful results of two migraine mutations. We noticed that L1670W, however not F1774S, decreased present density and that each mutations led to a dramatic enhance in persistent sodium currents, a depolarizing shift of the regular state-inactivation voltage-dependence, and a sooner restoration from inactivation.
The outcomes are per a serious gain-of operate impact underlying familial hemiplegic migraine 3. Our optimization technique will assist to characterize in an environment friendly method the impact in vitro of mutations of neuronal voltage-gated sodium channels.

Comparability of assorted RNA extraction strategies, cDNA preparation and isolation of calmodulin gene from a extremely melanized isolate of apple leaf blotch fungus Marssonina coronaria.

Marssonina coronaria causes apple blotch illness leading to extreme untimely defoliation, and is distributed in lots of main apple-growing areas on the earth. Efficient, dependable and high-quality RNA extraction is an indispensable process in any molecular biology research. No technique at present exists for RNA extraction from M. coronaria that produces a excessive amount of melanin-free RNA.
Due to this fact, we evaluated eight RNA extraction strategies together with handbook and business kits, to yield a adequate amount of high-quality and melanin-free RNA. Handbook strategies used right here resulted in low high quality and black coloured RNA pellets displaying the presence of melanin, regardless of all of the modifications employed to unique procedures.
Nevertheless, these strategies when coupled with clear up resulted in melanin-free RNA. Alternatively, all business kits used have been capable of yield high-quality melanin-free RNA having variable yields. TRIzol™ Reagent + RNA Clear & Concentrator™-5 and Ambion-PureLink® RNA Mini Package have been discovered to be the perfect strategies because the RNA extracted with these strategies from 15 day previous fungal tradition grown on strong medium have been freed from melanin with good yield.

Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat

4-20393 ea Ask for price

Bovine SALIVARY PAROTID 25 EA*

57142-2 25 EA
EUR 410.83

Rabbit SALIVARY PAROTID Y 25 EA*

41241-2 25 EA
EUR 318.39

Rabbit SALIVARY PAROTID MATR 25 EA*

41342-2 25 EA
EUR 318.39

Anti-Parotid secretory protein (mouse) antibody

STJ72465 100 µg
EUR 260

Frozen Tissue Section - Human Tumor: Parotid

T1235190 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Human anti-parotid duct antibody ELISA Kit

GA-E0467HM-48T 48T
EUR 289

Human anti-parotid duct antibody ELISA Kit

GA-E0467HM-96T 96T
EUR 466

Human anti-parotid duct antibody ELISA Kit

201-12-0451 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human anti-parotid duct antibody ELISA Kit

QY-E02562 96T
EUR 361

Mouse Parotid secretory protein, Psp ELISA KIT

ELI-16790m 96 Tests
EUR 865

Rat anti parotid duct antibody ELISA kit

E02A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit

E02A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit

E09A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total Protein from Human Tumor Tissue: Parotid

P1235190 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Tissue: Parotid

P3235190 0.1 mg
EUR 633
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Tissue: Parotid

R1235190-10 10 ug
EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Frozen Tissue Section - Human Adult Normal: Parotid

T1234190 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Parotid

T2234190 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Human anti parotid duct antibody ELISA kit

E01A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit

E01A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit

E01A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit

E04A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit

E03A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit

E07A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit

E06A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit

E08A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Pancreas Dissociation System 5 (Acinar, mouse parotid), Mouse

4-20375 ea Ask for price

Pancreas Dissociation System 6 (Acinar, rat parotid), Rat

4-20376 ea Ask for price

Parotid Dissociation System 1 (Epithelial), Mouse and Rat

4-20391 ea Ask for price

Anti-parotid secretory protein (mouse aa146-160) antibody

STJ72466 100 µg
EUR 359

Total Protein from Human Adult Normal Tissue: Parotid

P1234190 1 mg
EUR 356
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Adult Normal Tissue: Parotid

R1234190-50 50 ug
EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Parotid Tumor: Pleomorphic Adenoma

T2235190-5 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-192T 192 tests
EUR 1270
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-48 1 plate of 48 wells
EUR 520
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit

E05A2104-96 1 plate of 96 wells
EUR 685
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 5mL
EUR 106

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 415

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 445

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 309

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Evo™ cDNA Kit

M1164-100 Ask for price

Evo™ cDNA Kit

M1164-25 Ask for price

Novo? Transcriptome cDNA Kit

M1167-100
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25
EUR 441

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 415

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 847

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1211

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
EUR 134

amfiRivet cDNA Synthesis 2X Buffer

R5102-100 2x500ul
EUR 192

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 690.00
  • EUR 565.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 10 rxn
EUR 415

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 2 ug
EUR 445

Human Adult cDNA Tissue: Lung

HA-152 10 rxn
EUR 415

Human Adult cDNA Tissue: Skin

HA-218 10 rxn
EUR 415

Human Adult cDNA Tissue: Testis

HA-260 10 rxn
EUR 415

Human Tumor Tissue: Breast cDNA

HT05-090 10 rxn
EUR 415

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 224

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 544

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 337.75

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 142.36

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 711.77

Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
EUR 77.76

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 801
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen

C1236246Lcs 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 381

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]

CD510VB-1 >1 x 10^6 IFUs
EUR 786
RNA extracted by this improved methodology was utilized for RT-PCR, subsequent PCR amplification, and isolation of calmodulin gene sequences from M. coronaria and contaminated apple leaf items. These strategies are extra time efficient than conventional strategies and take solely an hour to finish. To our information, that is the primary report on the strategy of isolation of high-quality RNA for cDNA synthesis in addition to isolation of the calmodulin gene sequence from this fungus.

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