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Non-structural Proteins of Severe Fever With Thrombocytopenia Syndrome Virus Suppress RNA Synthesis in a Transcriptionally Active cDNA-Derived Viral RNA Synthesis System
Extreme fever with thrombocytopenia syndrome (SFTS) is an rising infectious illness attributable to the tick-borne SFTS bunyavirus (SFTSV) leading to a excessive fatality charge as much as 30%. SFTSV is a negative-strand RNA virus containing three single-stranded RNA genome segments designated as L, M, and S, which respectively, encode the RNA-dependent RNA polymerase (RdRp), glycoproteins Gn and Gc, and nucleoprotein (N) and non-structural proteins (NSs). NSs can type inclusion our bodies (IBs) in contaminated and transfected cells.
A earlier research has supplied a clue that SFTSV NSs could also be concerned in virus-like or viral RNA synthesis; nonetheless, the main points stay unclear. Our work described right here reveals that SFTSV NSs can downregulate virus-like RNA synthesis in a dose-dependent method inside a cDNA-derived viral RNA synthesis system, i.e., minigenome (-) and minigenome (+) methods primarily based on transfection, superinfection, and luciferase reporter exercise willpower; in the meantime, NSs additionally present a weak inhibitory impact on virus replication.
By utilizing co-immunoprecipitation (Co-IP) and RT-PCR mixed with site-directed mutagenesis, we discovered that NSs suppress virus-like RNA or virus replication by means of interacting with N however not with RdRp, and the detrimental regulatory impact correlates carefully with the IB construction it shaped however is just not related to its function of antagonizing host innate immune responses.
When the cytoplasmic construction of IB shaped by SFTSV NSs was disadvantaged, the inhibitory impact of NSs on virus-like RNA synthesis would weaken and even disappear.
Equally, we additionally evaluated different bandavirus NSs that can’t type IB in neither contaminated nor transfected cells, and the outcomes confirmed that the NSs of Heartland bandavirus (HRTV) didn’t present a big inhibitory impact on virus-like RNA synthesis inside a minigenome system. Our findings present experimental proof that SFTSV NSs take part in regulating virus-like or viral RNA synthesis and the detrimental impact could also be because of the NSs-N interplay.
Antimicrobial Peptides from Crops: A cDNA-Library Based mostly Isolation, Purification, Characterization Strategy and Elucidating Their Modes of Motion
Even in a pure ecosystem, vegetation are repeatedly threatened by numerous microbial ailments. To avoid wasting themselves from these numerous infections, vegetation construct a strong, multilayered immune system by means of their pure chemical compounds. Among the many a number of essential bioactive compounds possessed by vegetation’ immune methods, antimicrobial peptides (AMPs) rank within the first tier.
These AMPs are environmentally pleasant, anti-pathogenic, and don’t carry hurt to people. Antimicrobial peptides might be remoted in a number of methods, however recombinant protein manufacturing has change into more and more widespread in recent times, with the Escherichia coli expression system being probably the most extensively used.
Nevertheless, the efficacy of this expression system is compromised because of the problem of eradicating endotoxin from its system. Due to this fact, this evaluate suggests a high-throughput cDNA library-based plant-derived AMP isolation approach utilizing the Bacillus subtilis expression system. This technique might be carried out for large-scale screening of plant sources to categorise distinctive or homologous AMPs for the agronomic and utilized subject of plant research.
Moreover, this evaluate additionally focuses on the efficacy of plant AMPs, that are depending on their quite a few modes of motion and distinctive structural stability to operate in opposition to a variety of invaders. To conclude, the findings from this research will probably be helpful in investigating how novel AMPs are distributed amongst vegetation and supply detailed pointers for an efficient screening technique of AMPs.
Adapting 3′ Fast Amplification of CDNA Ends to Map Transcripts in Most cancers.
Maturation of eukaryotic mRNAs entails 3′ finish formation, which entails the addition of a poly(A) tail. With a purpose to map the three’ finish of a gene, the normal technique of alternative is 3′ speedy amplification of cDNA ends (3′ RACE). Protocols for 3′ RACE require the cautious design and number of nested primers throughout the 3′ untranslated area (3′ UTR) of the goal gene of curiosity.
Nevertheless, with a number of modifications the protocol can be utilized to incorporate your entire 3′ UTR and sequences throughout the open studying body (ORF), offering a extra complete image of the connection between the ORF and the three’ UTR.
That is along with identification of the polyadenylation sign (PAS), in addition to the cleavage and polyadenylation website supplied by standard 3′ RACE. Expanded 3′ RACE can detect uncommon 3′ UTRs, together with gene fusions throughout the 3′ UTR, and the sequence info can be utilized to foretell potential miRNA binding websites in addition to AU wealthy destabilizing components that will have an effect on the steadiness of the transcript.
Achieve of operate of sporadic/familial hemiplegic migraine-causing SCN1A mutations: Use of an optimized cDNA.
Introduction Familial hemiplegic migraine Three is an autosomal dominant headache dysfunction related to aura and transient hemiparesis, attributable to mutations of the neuronal voltage-gated sodium channel Nav1.1. Whereas a gain-of operate phenotype is mostly assumed to underlie familial hemiplegic migraine, this has not been totally explored. Certainly, a serious impediment in learning in vitro neuronal sodium channels is the problem in propagating and mutagenizing expression plasmids containing their cDNAs.
The intention of this work was to review the useful impact of two beforehand uncharacterized hemiplegic migraine inflicting mutations, Leu1670Trp (L1670W) and Phe1774Ser (F1774S). Strategies A novel SCN1A containing-plasmid was designed in silico and synthesized, and migraine mutations have been inserted on this background. Entire-cell patch clamp was carried out to research the useful properties of mutant Nav1.1 transiently expressed in Human Embryonic Kidney 293 cells.
Outcomes and conclusions We generated an optimized Nav1.1 expression plasmid that was very simple to deal with and used the novel plasmid to review the useful results of two migraine mutations. We noticed that L1670W, however not F1774S, decreased present density and that each mutations led to a dramatic enhance in persistent sodium currents, a depolarizing shift of the regular state-inactivation voltage-dependence, and a sooner restoration from inactivation.
The outcomes are per a serious gain-of operate impact underlying familial hemiplegic migraine 3. Our optimization technique will assist to characterize in an environment friendly method the impact in vitro of mutations of neuronal voltage-gated sodium channels.
Comparability of assorted RNA extraction strategies, cDNA preparation and isolation of calmodulin gene from a extremely melanized isolate of apple leaf blotch fungus Marssonina coronaria.
Marssonina coronaria causes apple blotch illness leading to extreme untimely defoliation, and is distributed in lots of main apple-growing areas on the earth. Efficient, dependable and high-quality RNA extraction is an indispensable process in any molecular biology research. No technique at present exists for RNA extraction from M. coronaria that produces a excessive amount of melanin-free RNA.
Due to this fact, we evaluated eight RNA extraction strategies together with handbook and business kits, to yield a adequate amount of high-quality and melanin-free RNA. Handbook strategies used right here resulted in low high quality and black coloured RNA pellets displaying the presence of melanin, regardless of all of the modifications employed to unique procedures.
Nevertheless, these strategies when coupled with clear up resulted in melanin-free RNA. Alternatively, all business kits used have been capable of yield high-quality melanin-free RNA having variable yields. TRIzol™ Reagent + RNA Clear & Concentrator™-5 and Ambion-PureLink® RNA Mini Package have been discovered to be the perfect strategies because the RNA extracted with these strategies from 15 day previous fungal tradition grown on strong medium have been freed from melanin with good yield.
Rat WS Salivary gland, Parotid cDNA-Oligo-dT |
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| RD-316-WS | Zyagen | 30 reactions | EUR 243 |
cDNA - Monkey (Rhesus) Normal Tissue: Parotid |
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| C1534190 | Biochain | 40 reactions | EUR 723 |
cDNA - Monkey (Cynomolgus) Normal Tissue: Parotid |
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| C1534190-Cy | Biochain | 40 reactions | EUR 723 |
Mouse C57 Salivary gland, Parotid cDNA-Random Primer |
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| MD-316-C57-RH | Zyagen | 30 reactions | EUR 280 |
Mouse CD1 Salivary gland, Parotid cDNA-Random Primer |
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| MD-316-HR | Zyagen | 30 reactions | EUR 243 |
Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Parotid, BioGenomics |
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| MBS652586-40Tests | MyBiosource | 40Tests | EUR 850 |
Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Parotid, BioGenomics |
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| MBS652586-5x40Tests | MyBiosource | 5x40Tests | EUR 3600 |
Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Parotid, BioGenomics |
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| MBS652322-40Tests | MyBiosource | 40Tests | EUR 850 |
Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Parotid, BioGenomics |
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| MBS652322-5x40Tests | MyBiosource | 5x40Tests | EUR 3600 |
Parotid Dissociation System 2 (Parotid), Mouse and Rat |
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| 4-20392 | CHI Scientific | ea | Ask for price |
Parotid Dissociation System 4 (Parotid acinar), Mouse and Rat |
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| 4-20394 | CHI Scientific | ea | Ask for price |
Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat |
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| 4-20393 | CHI Scientific | ea | Ask for price |
Dcpp1 (untagged) - Mouse demilune cell and parotid protein 1 (cDNA clone MGC:11676 IMAGE:3709995), (10ug) |
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| MC200699 | Origene Technologies GmbH | 10 µg | Ask for price |
Parotid Membrane Tumor Lysate |
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| XBL-10807 | ProSci | 0.1 mg | EUR 1023 |
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Description: Human parotid tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human parotid tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated parotid tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated parotid tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
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Parotid secretory protein Antibody |
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| abx431530-200ul | Abbexa | 200 ul | EUR 460.8 |
Parotid secretory protein Antibody |
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| abx431531-200ul | Abbexa | 200 ul | EUR 343.2 |
Parotid secretory protein Antibody |
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| abx431530-200l | Abbexa | 200 µl | EUR 387.5 |
Parotid secretory protein Antibody |
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| abx431531-200l | Abbexa | 200 µl | EUR 250 |
Pig Salivary, Parotid Total RNA |
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| PR-316 | Zyagen | 0.1mg | EUR 160 |
Sheep Salivary, Parotid Total RNA |
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| SR-316 | Zyagen | 0.1mg | EUR 160 |
Bovine Salivary, Parotid Total RNA |
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| BR-316 | Zyagen | 0.1mg | EUR 160 |
Pig Salivary, Parotid Total Protein |
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| PT-316 | Zyagen | 1mg | EUR 153 |
Pig Salivary, Parotid Frozen Sections |
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| PF-316 | Zyagen | 10 slides | EUR 261 |
Sheep Salivary, Parotid Total Protein |
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| ST-316 | Zyagen | 1mg | EUR 153 |
Bovine Salivary, Parotid Total Protein |
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| BT-316 | Zyagen | 1mg | EUR 153 |
Pig Salivary, Parotid Paraffin Sections |
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| PP-316 | Zyagen | 10 slides | EUR 240 |
Sheep Salivary, Parotid Frozen Sections |
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| SF-316 | Zyagen | 10 slides | EUR 261 |
anti-parotid duct antibody/ Rat anti- parotid duct antibody ELIS |
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| ELA-E0500r | Lifescience Market | 96 Tests | EUR 1063.2 |
57142-2 BV SALIVARY PAROTID 25 EA* |
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| 57142-2 | Pel-Freez | 25 EA | EUR 563 |
Bovine Salivary, Parotid Frozen Sections |
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| BF-316 | Zyagen | 10 slides | EUR 261 |
Sheep Salivary, Parotid Paraffin Sections |
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| SP-316 | Zyagen | 10 slides | EUR 240 |
Total RNA - Human Tumor Tissue: Parotid |
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| R1235190-10 | Biochain | 10 ug | EUR 480 |
Rat Salivary Gland, Parotid Total RNA* |
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| RR-316 | Zyagen | 0.05mg | EUR 160 |
Bovine Salivary, Parotid Paraffin Sections |
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| BP-316 | Zyagen | 10 slides | EUR 240 |
parotid secretory protein Immunizing Peptide |
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| MBS427531-01mg | MyBiosource | 0.1mg | EUR 225 |
Parotid secretory protein Immunizing Peptide |
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| MBS427602-01mg | MyBiosource | 0.1mg | EUR 225 |
Total Protein - Human Tumor Tissue: Parotid |
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| P1235190 | Biochain | 1 mg | EUR 542 |
Rat Salivary gland, Parotid Total Protein |
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| RT-316 | Zyagen | 1mg | EUR 153 |
Tissue, Total Protein, Human Tumor, Parotid |
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| MBS657391-1mg | MyBiosource | 1mg | EUR 820 |
Tissue, Total Protein, Human Tumor, Parotid |
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| MBS657391-5x1mg | MyBiosource | 5x1mg | EUR 3470 |
41241-2 RB SALIVARY PAROTID Y 25 EA* |
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| 41241-2 | Pel-Freez | 25 EA | EUR 263 |
Innovative Grade US Origin Bovine Parotid |
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| IGBOPTDF | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGBOPTDP | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGBOPTDS | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGBOPTDZ | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGCLFPTDF | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGCLFPTDP | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGCLFPTDS | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Innovative Grade US Origin Bovine Parotid |
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| IGCLFPTDZ | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Bovine Parotid |
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Frozen Tissue Section - Human Tumor: Parotid |
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| T1235190 | Biochain | 5 slides | EUR 363 |
Tissue, Section, Human Tumor, Parotid (Frozen) |
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| MBS640597-5Sections | MyBiosource | 5Sections | EUR 620 |
Tissue, Section, Human Tumor, Parotid (Frozen) |
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| MBS640597-5x5Sections | MyBiosource | 5x5Sections | EUR 2570 |
Parotid gland disease spectrum (parotidean cancer progression) tissue array |
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| OR801 | TissueArray | each | EUR 306 |
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Description: Parotid gland disease spectrum (parotidean cancer progression) tissue array, 80 cases/80 cores |
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Innovative Grade US Origin Porcine Parotid |
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| IGPCPTDF | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Porcine Parotid |
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Innovative Grade US Origin Porcine Parotid |
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| IGPCPTDP | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Porcine Parotid |
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Innovative Grade US Origin Porcine Parotid |
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| IGPCPTDS | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Porcine Parotid |
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Innovative Grade US Origin Porcine Parotid |
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| IGPCPTDZ | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Porcine Parotid |
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Innovative Grade US Origin Chicken Parotid |
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| IGCHPTDF | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Chicken Parotid |
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Innovative Grade US Origin Chicken Parotid |
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| IGCHPTDP | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Chicken Parotid |
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Innovative Grade US Origin Chicken Parotid |
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| IGCHPTDS | Innovative research | each | EUR 210 |
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Description: Innovative Grade US Origin Chicken Parotid |
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Innovative Grade US Origin Chicken Parotid |
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| IGCHPTDZ | Innovative research | each | EUR 133 |
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Description: Innovative Grade US Origin Chicken Parotid |
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Rat Salivary gland, Parotid Frozen Sections |
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| RF-316 | Zyagen | 10 slides | EUR 228 |
Rat WS Salivary Gland, Parotid Total RNA* |
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| RR-316-WS | Zyagen | 0.05mg | EUR 160 |
41342-2 RB SALIVARY PAROTID MATR 25 EA* |
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| 41342-2 | Pel-Freez | 25 EA | EUR 263 |
Membrane Protein - Human Tumor Tissue: Parotid |
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| P3235190 | Biochain | 0.1 mg | EUR 768 |
Tissue, Membrane Protein, Human Tumor, Parotid |
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| MBS639837-01mg | MyBiosource | 0.1mg | EUR 930 |
Tissue, Membrane Protein, Human Tumor, Parotid |
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| MBS639837-5x01mg | MyBiosource | 5x0.1mg | EUR 3955 |
Rat Salivary gland, Parotid Paraffin Sections |
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| RP-316 | Zyagen | 10 slides | EUR 228 |
Rat anti parotid duct antibody ELISA kit |
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| E01A10220 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat anti parotid duct antibody ELISA kit |
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| E02A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig anti parotid duct antibody ELISA kit |
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| E07A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig anti parotid duct antibody ELISA kit |
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| E07A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig anti parotid duct antibody ELISA kit |
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| E07A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog anti parotid duct antibody ELISA kit |
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| E08A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog anti parotid duct antibody ELISA kit |
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| E08A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog anti parotid duct antibody ELISA kit |
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| E08A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Parotid Tumor Lysate Membrane Fraction |
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| IHUPTDTLTM100UG | Innovative research | each | EUR 1742 |
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Description: Human Parotid Tumor Lysate Membrane Fraction |
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Human Parotid Tumor Lysate Membrane Fraction |
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| MBS8418016-01mg | MyBiosource | 0.1mg | EUR 2215 |
Human Parotid Tumor Lysate Membrane Fraction |
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| MBS8418016-5x01mg | MyBiosource | 5x0.1mg | EUR 9780 |
Goat anti parotid duct antibody ELISA kit |
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| E01A45139 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Goat anti parotid duct antibody ELISA kit |
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| E06A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat anti parotid duct antibody ELISA kit |
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| E06A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat anti parotid duct antibody ELISA kit |
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| E06A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti parotid duct antibody ELISA kit |
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| E01A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti parotid duct antibody ELISA kit |
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| E01A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human anti parotid duct antibody ELISA kit |
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| E01A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse anti parotid duct antibody ELISA kit |
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| E01A18961 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Mouse anti parotid duct antibody ELISA kit |
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| E03A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse anti parotid duct antibody ELISA kit |
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| E03A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse anti parotid duct antibody ELISA kit |
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| E03A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Sheep anti parotid duct antibody ELISA kit |
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| E01A97432 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Human anti parotid duct antibody ELISA kit |
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| E01A1456 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Mouse CD1 Salivary Gland, Parotid Total RNA |
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| MR-316 | Zyagen | 0.05mg | EUR 160 |
Mouse C57 Salivary Gland, Parotid Total RNA |
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| MR-316-C57 | Zyagen | 0.05mg | EUR 180 |
Human anti parotid duct antibody ELISA Kit |
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| MBS7218624-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Human anti parotid duct antibody ELISA Kit |
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| MBS7218624-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Human anti parotid duct antibody ELISA Kit |
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| MBS7218624-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Human anti parotid duct antibody ELISA Kit |
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| MBS7218624-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
Canine anti parotid duct antibody ELISA kit |
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| E01A62573 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Monkey anti parotid duct antibody ELISA kit |
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| E01A71286 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Rabbit anti parotid duct antibody ELISA kit |
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| E01A27707 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Bovine anti parotid duct antibody ELISA kit |
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| E01A80003 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Rabbit anti parotid duct antibody ELISA kit |
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| E04A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit anti parotid duct antibody ELISA kit |
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| E04A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit anti parotid duct antibody ELISA kit |
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| E04A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey anti parotid duct antibody ELISA kit |
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| E09A2104-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey anti parotid duct antibody ELISA kit |
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| E09A2104-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey anti parotid duct antibody ELISA kit |
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| E09A2104-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Total RNA - Human Adult Normal Tissue: Parotid |
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| R1234190-50 | Biochain | 50 ug | EUR 239 |
Rat WS Salivary gland, Parotid Total Protein |
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| RT-316-WS | Zyagen | 1mg | EUR 153 |
Rabbit Anti Parotid Duct Antibody ELISA Kit |
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| MBS7256617-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5685 |
Rabbit Anti Parotid Duct Antibody ELISA Kit |
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| MBS7256617-48StripWells | MyBiosource | 48-Strip-Wells | EUR 485 |
Rabbit Anti Parotid Duct Antibody ELISA Kit |
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| MBS7256617-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 3020 |
Rabbit Anti Parotid Duct Antibody ELISA Kit |
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| MBS7256617-96StripWells | MyBiosource | 96-Strip-Wells | EUR 690 |
OCPB00693-100UG - Parotid Membrane Tumor Lysate |
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| OCPB00693-100UG | Aviva Systems Biology | 0.1mg | EUR 979 |
Porcine anti parotid duct antibody ELISA kit |
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| E01A53856 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Chicken anti parotid duct antibody ELISA kit |
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| E01A88729 | BlueGene | 96T | EUR 700 |
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Description: ELISA |
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Mouse Balbc Salivary Gland, Parotid Total RNA |
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| MR-316-BLC | Zyagen | 0.05mg | EUR 180 |
Rat WS Salivary gland, Parotid Frozen Sections |
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| RF-316-WS | Zyagen | 10 slides | EUR 228 |
Mouse Parotid secretory protein (PSP) ELISA Kit |
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| abx540578-96tests | Abbexa | 96 tests | EUR 687.5 |
Mouse Parotid secretory protein, Psp ELISA KIT |
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| ELI-16790m | Lifescience Market | 96 Tests | EUR 1038 |
Mouse CD1 Salivary gland, Parotid Total Protein |
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| MT-316 | Zyagen | 1mg | EUR 153 |
Mouse BLC Salivary gland, Parotid Total Protein |
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| MT-316-BLC | Zyagen | 1mg | EUR 180 |
Mouse C57 Salivary gland, Parotid Total Protein |
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| MT-316-C57 | Zyagen | 1mg | EUR 180 |
Rat WS Salivary gland, Parotid Paraffin Sections |
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| RP-316-WS | Zyagen | 10 slides | EUR 228 |
Total Protein - Human Adult Normal Tissue: Parotid |
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| P1234190 | Biochain | 1 mg | EUR 404 |
Tissue, Total Protein, Human Adult Normal, Parotid |
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| MBS657549-1mg | MyBiosource | 1mg | EUR 710 |
Tissue, Total Protein, Human Adult Normal, Parotid |
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| MBS657549-5x1mg | MyBiosource | 5x1mg | EUR 2980 |
Mouse CD1 Salivary gland, Parotid Frozen Sections |
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| MF-316 | Zyagen | 10 slides | EUR 228 |
Mouse BLC Salivary gland, Parotid Frozen Sections |
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| MF-316-BLC | Zyagen | 10 slides | EUR 253 |
Mouse C57 Salivary gland, Parotid Frozen Sections |
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| MF-316-C57 | Zyagen | 10 slides | EUR 253 |
Frozen Tissue Section - Human Adult Normal: Parotid |
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| T1234190 | Biochain | 5 slides | EUR 377 |
Tissue, Total RNA, Human Tumor, Parotid, BioGenomics |
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| MBS638750-001mg | MyBiosource | 0.01mg | EUR 655 |
Tissue, Total RNA, Human Tumor, Parotid, BioGenomics |
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| MBS638750-5x001mg | MyBiosource | 5x0.01mg | EUR 2735 |
Tissue, Section, Human Adult Normal, Parotid (Frozen) |
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| MBS640015-5Sections | MyBiosource | 5Sections | EUR 635 |
Tissue, Section, Human Adult Normal, Parotid (Frozen) |
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| MBS640015-5x5Sections | MyBiosource | 5x5Sections | EUR 2640 |
Mouse CD1 Salivary gland, Parotid Paraffin Sections |
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| MP-316 | Zyagen | 10 slides | EUR 228 |
Mouse BLC Salivary gland, Parotid Paraffin Sections |
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| MP-316-BLC | Zyagen | 10 slides | EUR 253 |
RNA extracted by this improved methodology was utilized for RT-PCR, subsequent PCR amplification, and isolation of calmodulin gene sequences from M. coronaria and contaminated apple leaf items. These strategies are extra time efficient than conventional strategies and take solely an hour to finish. To our information, that is the primary report on the strategy of isolation of high-quality RNA for cDNA synthesis in addition to isolation of the calmodulin gene sequence from this fungus.
