fimH Antibody

Product details

Full Product Name

Rabbit Anti-Escherichia coli Polyclonal Antibody (K12 strain) fimH

Uniprot No: P08191

Target names: fimH

Alternative names

fimH antibody; b4320 antibody; JW4283 Fimbrin D-mannose type 1 specific adhesin antibody; FimH protein antibody

Raised: Rabbit

Species Reactivity: Escherichia coli

 Immunogenic

Recombinant fimbrin D-mannose specific adhesin from Escherichia coli Type 1 (fimH) (22-300AA)

Immunogenic species: Escherichia coli (strain K12)

Conjugated: not conjugated

Clonality: Polyclonal

Isotype: IgG

Purification method: > 95%, purified protein G

Concentration

It differs from different batches. Please contact us to confirm.

Buffer

  • Preservative: 0.03% Proclin 300
  • Constituents: 50% glycerol, 0.01 M PBS, pH 7.4

Form: Liquid

Tested applications

ELISA, WB (ensure antigen identification)

Protocols: ELISA protocol

Storage

On receipt, store at -20 ┬░ C or -80 ┬░ C. Avoid repeated freezing.

Delivery time

Basically, we can ship the products in 1-3 business days after receiving your orders. Delivery time may differ from different purchase forms or locations, please consult your local dealers for specific delivery times.

Target background

  • Function (From Uniprot)

Participates in the regulation of the length and mediation of the adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin is responsible for binding to D-mannose. It is placed laterally at intervals in the structure of type 1 fimbriae. FimF and FimG are required to integrate FimH into the fimbriae.

  • Functional genetic references
  1. Conformational change of the bacterial FimH adhesin in the absence of the regulatory domain. PMID: 29180452
  2. Evolutionary adjustment of conformational sets in FimH during host-pathogen interactions. PMID: 28246638
  3. A mutant lacking the type 1 pilus-associated adhesin FimH showed somewhat reduced persistence within the gut. PMID: 29311232
  4. The authors demonstrated that FimH residues E50 and T53 are crucial for adhesion under flow conditions. PMID: 27816993
  5. In the absence of pulling force, the pilin domain of FimH allosterically accelerates the spontaneous dissociation of the ligand from the lectin domain of FimH by 100,000 times. PMID: 26948702
  6. Mouse urothelium responds to type 1 fimbriated UPEC adhesion by activating the dual ligand/receptor system, one between FimH adhesin and uroplakin Ia and the other between lipopolysaccharide and Tlr4. PMID: 26549759
  7. Luteolin decreased UPEC binding and invasion into bladder epithelial cells by negatively regulating the expression of the adhesin gene fimH PMID: 25051393
  8. The data indicate that thiazolylaminomannosides prevented bacterial attachment to the gut by blocking the bacterial adhesin FimH. PMID: 23795713
  9. Promising new vaccine combinations based on the FliC antigen against urinary tract infections caused by uropathogenic Escherichia coli. PMID: 23220068
  10. FimH elicits an immune response that enhances Escherichia coli cell adhesion. PMID: 21768279

Subcellular location: Fimbrium

Protein families: Fimbrial protein family

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