Feline Rapid FPV Ag

1. Use of the product

Cat fever, also known as feline panleukopenia and feline infectious gastroenteritis, is a highly contagious acute feline disease. The causative agent is feline parvovirus (FPV), which is highly homologous to canine parvovirus type 2. The virus primarily infects cats, and domestic cats and feral cats can become infected. It is highly contagious, has an acute onset, and has a high mortality rate.

Cat plague is generally transmitted through bodily fluids, faeces, contaminants, and even fleas from infected animals, with susceptible animals becoming infected primarily through direct or indirect contact. This product uses an immunochromatographic test of feline faeces or vomit samples to quickly detect if a cat is infected with feline panleukopenia.

2. Principle

This product uses rapid immunochromatography to detect FPV antigens. After adding the diluted sample to the “S” hole on the test card, if FPV antigen is present in the sample, the antigen will specifically bind to the colloidal gold-labelled FPV monoclonal antibody and the complex will move along the test card. chromatographic membrane. and be chromatographed.

The FPV monoclonal antibody precoated on the membrane is captured and a wine-red detection line is formed at position T of the cartridge. If there is no FPV antigen in the sample, no visible lines will form on the T line. In addition, the system has also drawn a C line to verify that the test is valid; either negative or positive, the line must be coloured, otherwise, it will be invalid.

3. Components

  • FPV Ag Test Card (including 1 FPV Ag test cassette, 1 dropper, 1 desiccant) 10 pieces
  • Sample buffer (1ml/tube) 10 tubes
  • Swab 10 pieces
  • Instruction 1 piece

4. Test procedure

  • Please read the manual carefully and return the test card, and sample at room temperature 15~25℃ before use.
  • Specimen Collection: Use a cotton swab to collect a sample of freshly excreted stool or vomit, or take a sample directly from the rectum. Immediately insert the cotton swab with the discharge into the sample tube containing the sample buffer and mix evenly so that the sample dissolves in the buffer as much as possible.
  • Open the package, take out the test card, and put it on the desk flat.
  • Use the supplied dropper to absorb the diluted sample, and drop 3~4 drops (about 100ul) into the “S” hole of the test card.
  • Read the result in 5~10 minutes, the result is invalid beyond 15 minutes.

5. Judgment of the result

  • Positive (+): Both the T line and the C line show a wine-red colour reaction, which means that there is FPV antigen in the sample.
  • Negative (-): no colour reaction in the test line (T line), only the control line (C line) shows a wine red colour reaction, which means there is no FPV antigen in the sample .
  • Invalid (X): No colour reaction on the C line, it means a wrong operation or the test card is invalid, please retest.

6. Specifications, Storage and Stability.

  • Package: 10T/box
  • Store at 4~30℃ in a cool, dark and dry place, not frozen.
  • Shelf life: 18 months.

7. Precautions

  • Please use the test card within the shelf life and within 1 hour after opening.
  • Avoid direct sunlight and direct blowing of the fan during the test.
  • Try not to touch the white film surface of the non-plastic part of the test card.
  • Sample droppers should not be mixed to avoid cross-contamination.
  • Do not use sample buffers that are not supplied with this reagent.
  • The used test card must be treated as microbiological dangerous goods.
  • When the content of the detected substance is very high, the T line may appear, and then the red colour will gradually fade or even disappear. It is recommended to dilute the sample 10 times before the test so that the T line can be stable.

8. Limitations of the application

This product is an immunological diagnostic kit, which is only used to provide qualitative test results for clinical testing of pet diseases. If you have any doubts about the test results, please use other diagnostic methods (such as RT-PCR, Western blot, etc.) to analyze the samples further. For pathology analysis, see your local pet doctor.

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