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Complete genome sequence and construction of an infectious full-length cDNA clone of a cucumber vein yellowing virus (CVYV) isolate from Portugal
Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus within the household Potyviridae. Within the Nationwide Middle for Biotechnology Data (NCBI) database, three full genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) can be found.
On this examine, we report the whole sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) together with the development of an infectious full-length cDNA clone by way of Gibson meeting. The sequence of CVYV Portugal reveals the closest relationship to a CVYV isolate from Spain (genome, 99.7% id; polyprotein, 99.7% id).
The CVYV full-length cDNA clone was launched by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, leading to signs resembling these of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This primary report confirming the infectivity of a CVYV cDNA clone supplies the chance to check gene capabilities in a constant genomic background.
Tick CAPA propeptide cDNAs and receptor exercise of endogenous tick pyrokinins and analogs: in direction of discovering pyrokinin operate in ticks
Pyrokinins (PKs) are pleiotropic neuropeptides with important roles in invertebrate physiology. Though capabilities of PKs are identified in bugs, there’s a lack of awareness of PK-encoding genes and PKs capabilities in ticks. Herein the primary tick cDNAs of the aptitude (capa) gene had been cloned from the southern cattle tick, Rhipicephalus microplus (Acari: Ixodidae), and the blacklegged tick, Ixodes scapularis.
Every cDNA encoded one periviscerokinin and 5 completely different pyrokinins. Two PKs had been equivalent in sequence within the two species. The three PKs distinctive to R. microplus (Rhimi-CAPA-PK1, -PK2, and -PK5) had been examined on the recombinant R. microplus pyrokinin receptor utilizing a calcium bioluminescence assay. The Rhimi-CAPA-PKs acted as agonists with EC50s starting from 101-207 nM.
Twenty PK analogs designed for enhanced bioavailability and biostability had been examined on the receptor. 5 of those had been designed primarily based on the sequences of the three distinctive Rhimi-CAPA-PKs. Eight PK analogs had been additionally agonists; 4 of them had been full agonists that exhibited comparable efficacy to the native Rhimi-CAPA-PKs, with EC50 starting from 401 nM-1.9 µM.
The structure-activity relationships (SAR) of all analogs had been analyzed. Our outcomes urged {that a} positively charged, fundamental lysine on the variable place X of the PK energetic core (FXPRLamide) conferred enhanced affinity to the analogs of their interplay with the tick receptor. These analogs are promising instruments to elucidate the pyrokinin operate in ticks in vivo as these analogs are anticipated to have extended hemolymph residence time compared to the native peptides.
Interleukin-17A Peptide Aptamers with an Surprising Binding Moiety Chosen by cDNA Show below Heterogenous Situations
Peptide-based medicine are a gorgeous new modality of therapeutics, and in vitro choice from a large-scale library is a robust approach to determine new lead sequences. In typical screenings, peptide specificity and stability in physiological heterogenous environments aren’t evaluated, which typically makes subsequent optimization tough.
Right here we present that choice utilizing a cDNA show system may be carried out in a excessive share of serum and that this is likely to be an possibility to pick out molecules with excessive efficiency and stability in a organic context.
Particularly, we selected interleukin-17A as a goal protein and carried out in vitro number of cyclic peptide aptamers from a library of roughly 1012 members within the presence of serum. The chosen molecules had nanomolar affinity to the goal and had been steady in serum. Curiously, we discovered {that a} element of the DNA linker that linked the peptide and cDNA could play a pivotal function in goal binding.
Purification, Characterization, and cDNA Cloning of a Outstanding β-Glucosidase from the Intestine of the Xylophagous Cockroach Panesthia angustipennis spadica
On this examine, a β-glucosidase (PaBG1b) with excessive particular exercise was purified from intestine extracts of the wood-feeding cockroach Panesthia angustipennis spadica utilizing Superdex 75 gel filtration chromatography and Excessive-Lure phenyl hydrophobic chromatography.
The protein was purified 14-fold to a single band recognized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, with an obvious molecular mass of 56.7 kDa. The precise exercise of the purified enzyme was 708 μmol/min/mg protein utilizing cellobiose as substrate. To the most effective of our information, that is the very best particular exercise reported amongst β-glucosidases thus far.
The purified PaBG1b confirmed optimum exercise at pH 5.zero and retained greater than 65 % of the exercise between pH 4.zero and 6.5. The exercise was steady as much as 50 °C for 30 min.
Kinetic research on cellobiose revealed that the Ok m was 5.three mM, and the V max was 1,020 μmol/min/mg. The inner amino acid sequence of PaBG1b was analyzed, and two steady sequences (a complete of 39 amino acids) of the C-terminal area had been elucidated.
Based mostly on these amino acid sequences, a full-length cDNA (1,552 bp) encoding 502 amino acids was remoted. The encoded protein confirmed excessive similarity to β-glucosidases from glycoside hydrolase household 1.
Thus, the present examine demonstrated the potential of PaBG1b for software in enzymatic biomass-conversion as a donor gene for heterologous recombination of cellulase-producing brokers (fungi or micro organism) or an additive enzyme for cellulase merchandise primarily based on the high-performance of PaBG1b as a digestive enzyme in cockroaches.
Cloning and expression sample of phosphate transporter 1;1 cDNA sequence from Spirodela polyrrhiza
Spirodela polyrrhiza is a floating plant extensively utilized in biomass utilization and eutrophication phytoremediation. It turns into a typical aquatic plant in all places with the more and more critical eutrophication. It has been reported that S. polyrrhiza has a very good impact on the remediation of eutrophication water.
With the intention to examine the absorption and transportation of phosphorus in S. polyrrhiza, we extracted RNA from S. polyrrhiza after which reverse transcribed it into cDNA, which was used as a template to amplify a particular fragment.
The complete-length sequence of the open studying body (ORF) was 1 620 bp, encoding 539 amino acids, named SpPHT1;1, and the accession quantity in GenBank was MN720003. Bioinformatical evaluation confirmed that SpPHT1;1 had no intron.
The protein it encoded was a steady, hydrophobic protein with 11 transmembrane domains. SpPHT1;1 construction was much like that of main facilitator superfamily (MFS) superfamily members. The cluster evaluation confirmed that SpPHT1;1 was intently associated to ZMPHT2 in maize and SBPHT1-Eight in sorghum.
So, it’d belong to plant PHT1 household. The expression of SpPHT1;1 in leaf was considerably greater than that of root below regular phosphorus situation. Low phosphorus situation might promote gene expression, and the relative expression degree of SpPHT1;1 arrived on the peak at 48 h each in root and leaf.
Total RNA - Rat Normal Tissue: Kidney |
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| R1434142-50 | Biochain | 50 ug | EUR 156 |
Genomic DNA - Rat Normal Tissue: Kidney |
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| D1434142 | Biochain | 100 ug | EUR 329 |
Rat normal kidney tissue array |
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| TR071a | TissueArray | each | EUR 48 |
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Description: Rat normal kidney tissue array, 6 cases/24 cores, replacing TR071 |
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Matched Pair - cDNA - Human Primary Tumor and Normal Tissue: Kidney |
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| C8235142-PP | Biochain | 10 reactions x2 | EUR 583 |
Normal kidney and normal adjacent kidney tissue array |
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| KDN241a | TissueArray | each | EUR 138 |
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Description: Normal kidney and normal adjacent kidney tissue array, 24 cases/ 24 cores |
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Kidney cancer tissue array with normal Kidney tissue |
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| KD2082a | TissueArray | each | EUR 474 |
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Description: Kidney cancer tissue array with normal Kidney tissue, including TNM and pathology grade, 96 cases/192 cores, replacing KD2082 |
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Kidney Tissue Slide (Normal) |
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| 10-401-10um | ProSci | 10 um | EUR 241.8 |
Kidney Tissue Slide (Normal) |
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| 10-401-4um | ProSci | 4 um | EUR 216.6 |
Normal kidney tissue array |
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| KD803 | TissueArray | each | EUR 354 |
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Description: Normal kidney tissue array, replacing BN07012, 80 cases/80 cores |
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Normal kidney tissue array |
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| KDN242 | TissueArray | each | EUR 72 |
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Description: Normal kidney tissue array, 24 cases/24 cores |
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Kidney Tissue Slide (Normal) |
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| MBS154338-10um | MyBiosource | 10um | EUR 210 |
Kidney Tissue Slide (Normal) |
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| MBS154338-4um | MyBiosource | 4um | EUR 195 |
Kidney Tissue Slide (Normal) |
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| MBS154338-5x10um | MyBiosource | 5x10um | EUR 920 |
Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics |
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| MBS652496-40Tests | MyBiosource | 40Tests | EUR 680 |
Tissue cDNA, First Strand, Mouse Adult Normal, Kidney, BioGenomics |
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| MBS652496-5x40Tests | MyBiosource | 5x40Tests | EUR 2835 |
Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics |
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| MBS652261-40Tests | MyBiosource | 40Tests | EUR 680 |
Tissue cDNA, First Strand, Human Adult Normal, Kidney, BioGenomics |
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| MBS652261-5x40Tests | MyBiosource | 5x40Tests | EUR 2835 |
Kidney Tissue Lysate (Normal) |
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| 1706-02 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Normal) |
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| 1706-03 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Normal) |
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| 1706-04 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Kidney Tissue Lysate (Normal) |
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| 1706-05 | ProSci | 0.1 mg | EUR 260.7 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
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Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics |
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| MBS652439-40Tests | MyBiosource | 40Tests | EUR 680 |
Tissue cDNA, First Strand, Canine Adult Normal, Kidney, BioGenomics |
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| MBS652439-5x40Tests | MyBiosource | 5x40Tests | EUR 2835 |
Rat Tissue Lysate Kidney Medulla Normal |
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| GWB-F5DEBF | GenWay Biotech | 0.15 mg | Ask for price |
Kidney cancer tissue array with adjacent normal kidney tissue |
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| BC07015b | TissueArray | each | EUR 168 |
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Description: Kidney cancer tissue array with adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 72 cases/72 cores, replacing BC07015a |
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Total RNA - Mouse Normal Tissue: Kidney |
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| R1334142-50 | Biochain | 50 ug | EUR 156 |
Genomic DNA - Mouse Normal Tissue: Kidney |
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| D1334142 | Biochain | 100 ug | EUR 329 |
Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics |
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| MBS652552-40Tests | MyBiosource | 40Tests | EUR 680 |
Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics |
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| MBS652552-5x40Tests | MyBiosource | 5x40Tests | EUR 2835 |
Total Protein - Mouse Normal Tissue: Kidney |
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| P1334142 | Biochain | 1 mg | EUR 216 |
Tissue Lysate (Normal) Human Kidney |
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| GWB-FEAC28 | GenWay Biotech | 0.15 mg | Ask for price |
Kidney cancer tissue array with matched adjacent normal kidney tissue |
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| KD901a | TissueArray | each | EUR 258 |
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Description: Kidney cancer tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 40 cases/90 cores, replacing KD901 |
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Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics |
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| MBS652401-40Tests | MyBiosource | 40Tests | EUR 680 |
Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Kidney, BioGenomics |
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| MBS652401-5x40Tests | MyBiosource | 5x40Tests | EUR 2835 |
Kidney tumour with normal tissue array |
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| KD2001 | TissueArray | each | EUR 474 |
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Description: Kidney tumour with normal tissue array, including Clear cell carcinoma (CCRCC), Sarcomatoid carcinoma, Papillary renal cell carcinoma (PRCC), Chromopobe carcinoma(crcc), Invasive low grade urothelial carcinoma, with Isup Grade, TNM/Stage (AJCC 8th edition), 100 cases/200 cores (core size 1.0mm),replacing BC07114 |
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Total RNA - Human Adult Normal Tissue: Kidney |
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| R1234142-50 | Biochain | 50 ug | EUR 221 |
Rat Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
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| 9-80263 | CHI Scientific | 1 x 100 ml | Ask for price |
Kidney cancer tissue array with normal tissues |
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| KD2083 | TissueArray | each | EUR 474 |
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Description: Kidney cancer tissue array with normal tissues, with stage and grade info, 69 cases/208 cores |
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Kidney cancer tissue array with normal tissues |
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| KD721 | TissueArray | each | EUR 168 |
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Description: Kidney cancer tissue array with normal tissues, 24 cases/72 cores |
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Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics |
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| MBS652533-2Panels | MyBiosource | 2Panels | EUR 805 |
Tissue cDNA (Matched Pairs), Human Primary Tumor and Normal, Kidney, BioGenomics |
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| MBS652533-5x2Panels | MyBiosource | 5x2Panels | EUR 3400 |
Mouse Normal Tissue: Kidney Whole Cell Lysate |
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| MNK-100 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Mouse Normal Tissue: Kidney Whole Cell Lysate |
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| MNK-50 | Alpha Diagnostics | 50 ug | EUR 196.8 |
Total RNA - Monkey (Rhesus) Normal Tissue: Kidney |
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| R1534142-50 | Biochain | 50 ug | EUR 229 |
Kidney tumor tissue array with normal tissue as control |
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| KD961 | TissueArray | each | EUR 258 |
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Description: Kidney tumor tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
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Total Protein - Human Adult Normal Tissue: Kidney |
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| P1234142 | Biochain | 1 mg | EUR 216 |
Kidney cancer tissue array with normal tissue as control |
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| KD962 | TissueArray | each | EUR 258 |
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Description: Kidney cancer tissue array with normal tissue as control, including TNM, clinical stage and pathology grade, 48 cases/96 cores |
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Total RNA - Monkey (Cynomolgus) Normal Tissue: Kidney |
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| R1534142-Cy | Biochain | 50 ug | EUR 229 |
Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics |
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| MBS638687-005mg | MyBiosource | 0.05mg | EUR 435 |
Tissue, Total RNA, Rat Adult Normal, Kidney, BioGenomics |
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| MBS638687-5x005mg | MyBiosource | 5x0.05mg | EUR 1735 |
Membrane Protein - Human Adult Normal Tissue: Kidney |
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| P3234142 | Biochain | 0.1 mg | EUR 311 |
Adjacent normal kidney tissue and cancer tissue array |
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| BN07011b | TissueArray | each | EUR 203 |
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Description: Adjacent normal kidney tissue and cancer tissue array, including pathology grade, TNM and clinical stage, 20 cases/54 cores, replacing BN07011 |
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Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue |
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| KD701a | TissueArray | each | EUR 234 |
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Description: Kidney cancer (grade I) tissue array with matched adjacent normal kidney tissue, including TNM, clinical stage and pathology grade, 35 cases/70 cores, replacing KD701 |
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FFPE Total RNA - Human Adult Normal Tissue: Kidney |
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| R2234142 | Biochain | 1 ug | EUR 744 |
Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics |
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| MBS654611-01mg | MyBiosource | 0.1mg | EUR 575 |
Tissue, Genomic DNA, Rat Adult Normal, Kidney, BioGenomics |
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| MBS654611-5x01mg | MyBiosource | 5x0.1mg | EUR 2440 |
Kidney cancer tissue array with matched adjacent normal tissue |
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| KD321a | TissueArray | each | EUR 198 |
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Description: Kidney cancer tissue array with matched adjacent normal tissue, including TNM, clinical stage and pathology ISUP grade, 16 cases/32 cores, replacing KD321 |
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Kidney cancer with matched adjacent normal kidney tissue array |
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| KD603 | TissueArray | each | EUR 204 |
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Description: Kidney cancer with matched adjacent normal kidney tissue array, containing metastatic clear cell carcinoma, including pathology grade, TNM and clinical stage (reference AJCC 8th version), 33 cases/61 cores |
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Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue |
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| Kid-060Sur01 | TissueArray | each | EUR 954 |
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Description: Kidney cancer tissue array with matched normal adjacent or caner adjacent kidney tissue, including TNM, ISUP grade and survival data, 30 cases/60 cores |
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Human Adult Kidney (Normal) Whole tissue lysate |
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| HAL-1305 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Monkey (Cyno.) Normal Kidney Whole tissue lysate |
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| MCL-1190 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Tissue, Total Protein, Mouse Adult Normal, Kidney |
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| MBS657420-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Mouse Adult Normal, Kidney |
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| MBS657420-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Tissue, Total Protein, Human Adult Normal, Kidney |
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| MBS657589-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Human Adult Normal, Kidney |
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| MBS657589-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Monkey (Rhesus) Normal Kidney Whole tissue lysate |
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| MRL-1301 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Frozen Tissue Section - Human Adult Normal: Kidney |
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| T1234142 | Biochain | 5 slides | EUR 377 |
Tissue, Section, Human Adult Normal, Kidney (Frozen) |
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| MBS640178-5Sections | MyBiosource | 5Sections | EUR 635 |
Tissue, Section, Human Adult Normal, Kidney (Frozen) |
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| MBS640178-5x5Sections | MyBiosource | 5x5Sections | EUR 2640 |
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney |
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| MBS640973-1Set | MyBiosource | 1Set | EUR 650 |
Tissue Set, BioAssay, DNA, RNA, Protein, Rat Adult Normal, Kidney |
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| MBS640973-5x1Set | MyBiosource | 5x1Set | EUR 2970 |
Kidney Tumor Tissue Array - 12 cases of kidney cancer paired with adjacent normal tissues |
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| Z7020053 | Biochain | 5 slides | EUR 1125.6 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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Paraffin Tissue Section - Human Adult Normal: Kidney |
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| T2234142 | Biochain | 5 slides | EUR 204 |
Tissue, Section, Human Adult Normal, Kidney (Paraffin) |
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| MBS640009-5Sections | MyBiosource | 5Sections | EUR 460 |
Tissue, Section, Human Adult Normal, Kidney (Paraffin) |
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| MBS640009-5x5Sections | MyBiosource | 5x5Sections | EUR 1925 |
Tissue, Membrane Protein, Human Adult Normal, Kidney |
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| MBS639828-01mg | MyBiosource | 0.1mg | EUR 570 |
Tissue, Membrane Protein, Human Adult Normal, Kidney |
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| MBS639828-5x01mg | MyBiosource | 5x0.1mg | EUR 2345 |
Human Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
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| 9-80075 | CHI Scientific | 1 x 100 ml | Ask for price |
Mouse Kidney Tissue Preparation Buffer 2: Normal Kidney Epithelial Cells |
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| 9-80171 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Kidney Tissue Preparation Buffer 6: Normal Renal Firbroblasts |
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| 9-80267 | CHI Scientific | 1 x 100 ml | Ask for price |
Multiple Kidney cancer tissue array with normal tissue control from autopsy |
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| KD483 | TissueArray | each | EUR 168 |
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Description: Multiple Kidney cancer tissue array with normal tissue control from autopsy, 48 cases/48 cores, with stage and grade data |
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Rat Normal Kidney tissue (single section per slide) (5 slides/pack) |
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| RaFPT007 | TissueArray | 5 slides | EUR 150 |
Kidney Tissue Slides, Normal Rat Paraffin Sections, 5 slides/pack |
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| TS-R5405 | IHC World | - | EUR 145 |
Matched Pair - DNA - Human Primary Tumor and Normal Tissue: Kidney |
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| D8235142-PP-10 | Biochain | 2x10 ug | EUR 478 |
Total RNA - Human Adult Normal Tissue 5 Donor Pool: Kidney |
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| R1234142-P | Biochain | 50 ug | EUR 443 |
cDNA - Rat Normal Tissue: Lung |
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| C1434152 | Biochain | 40 reactions | EUR 424 |
Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics |
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| MBS638539-005mg | MyBiosource | 0.05mg | EUR 435 |
Tissue, Total RNA, Mouse, Adult Normal, Kidney, BioGenomics |
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| MBS638539-5x005mg | MyBiosource | 5x0.05mg | EUR 1735 |
Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics |
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| MBS638594-005mg | MyBiosource | 0.05mg | EUR 495 |
Tissue, Total RNA, Human Adult Normal, Kidney, BioGenomics |
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| MBS638594-5x005mg | MyBiosource | 5x0.05mg | EUR 1995 |
cDNA - Rat Normal Tissue: Brain |
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| C1434035 | Biochain | 40 reactions | EUR 424 |
cDNA - Rat Normal Tissue: Colon |
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| C1434090 | Biochain | 40 reactions | EUR 636 |
cDNA - Rat Normal Tissue: Heart |
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| C1434122 | Biochain | 40 reactions | EUR 424 |
cDNA - Rat Normal Tissue: Liver |
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| C1434149 | Biochain | 40 reactions | EUR 424 |
cDNA - Rat Normal Tissue: Rectum |
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| C1434206 | Biochain | 40 reactions | EUR 636 |
cDNA - Rat Normal Tissue: Spleen |
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| C1434246 | Biochain | 40 reactions | EUR 424 |
cDNA - Rat Normal Tissue: Testis |
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| C1434260 | Biochain | 40 reactions | EUR 424 |
High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue |
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| KD208 | TissueArray | each | EUR 546 |
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Description: High density tissue array (70 cases/208 cores) of kidney cancer and normal tissue |
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cDNA - Rat Normal Tissue: Adipose |
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| C1434003 | Biochain | 40 reactions | EUR 636 |
cDNA - Rat Normal Tissue: Bladder |
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| C1434010 | Biochain | 40 reactions | EUR 424 |
cDNA - Rat Normal Tissue: Stomach |
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| C1434248 | Biochain | 40 reactions | EUR 424 |
Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics |
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| MBS654413-01mg | MyBiosource | 0.1mg | EUR 575 |
Tissue, Genomic DNA, Mouse Adult Normal, Kidney, BioGenomics |
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| MBS654413-5x01mg | MyBiosource | 5x0.1mg | EUR 2440 |
Genomic DNA - Human Adult Normal Tissue: Kidney, from a single donor |
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| D1234142 | Biochain | 100 ug | EUR 329 |
cDNA - Rat Normal Tissue: Placenta |
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| C1434200 | Biochain | 40 reactions | EUR 636 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Kidney |
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| R8235142-PP-10 | Biochain | 2x10 ug | EUR 500 |
Mouse Kidney Tissue Preparation Buffer 3: Normal Podocytes |
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| 9-80172 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Kidney Tissue Preparation Buffer 1: Normal Glomerular Endothelial Cells |
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| 9-80262 | CHI Scientific | 1 x 100 ml | Ask for price |
Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue |
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| KD242 | TissueArray | each | EUR 48 |
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Description: Multiple Kidney cancer test tissue array with unmatched normal adjacent tissue, 10 cases/24 cores |
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Kidney cancer with matched cancer adjacent normal tissue test array |
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| KD241 | TissueArray | each | EUR 48 |
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Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores |
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Kidney cancer with matched cancer adjacent normal tissue test array |
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| KD241a | TissueArray | each | EUR 66 |
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Description: Kidney cancer with matched cancer adjacent normal tissue test array, including TNM and clinical stage, 6 cases/24 cores, replacing KD241 |
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Rat Kidney Tissue Preparation Buffer 5: Normal Renal Artery Endothelial Cells |
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| 9-80266 | CHI Scientific | 1 x 100 ml | Ask for price |
Rat Kidney Tissue Preparation Buffer 8: Normal Renal Tubular Epithelial Cells |
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| 9-80269 | CHI Scientific | 1 x 100 ml | Ask for price |
Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics |
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| MBS638547-005mg | MyBiosource | 0.05mg | EUR 485 |
Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Kidney, BioGenomics |
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| MBS638547-5x005mg | MyBiosource | 5x0.05mg | EUR 1960 |
Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Kidney |
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| P8235142-PP | Biochain | 0.2 mg x2 | EUR 716 |
Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney |
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| MBS639416-1Kit | MyBiosource | 1Kit | EUR 730 |
Tissue Set, BioAssay, DNA, RNA, Protein, Human Adult Normal, Kidney |
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| MBS639416-5x1Kit | MyBiosource | 5x1Kit | EUR 3075 |
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney |
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| MBS640995-1Set | MyBiosource | 1Set | EUR 650 |
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Kidney |
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| MBS640995-5x1Set | MyBiosource | 5x1Set | EUR 2970 |
Human Kidney Tissue Preparation Buffer 6: Normal Renal Podocytes |
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| 9-80079 | CHI Scientific | 1 x 100 ml | Ask for price |
cDNA - Rat Normal Tissue: Skeletal Muscle |
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| C1434171 | Biochain | 40 reactions | EUR 424 |
cDNA - Rat Normal Tissue: Small Intestine |
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| C1434226 | Biochain | 40 reactions | EUR 636 |
Human Kidney Tissue Preparation Buffer 4: Normal Renal Firbroblasts |
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| 9-80077 | CHI Scientific | 1 x 100 ml | Ask for price |
Mouse Kidney Tissue Preparation Buffer 5: Normal Renal Firbroblasts |
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| 9-80174 | CHI Scientific | 1 x 100 ml | Ask for price |
Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma |
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| KD951a | TissueArray | each | EUR 1434 |
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Description: Kidney cancer tissue array with matched normal adjacent tissue and metastatic carcinoma, with TNM, clinical stage, pathology grade and survival data, 32 cases/95 cores,replacing KD951 |
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Kidney Tissue Slides, Normal Human Paraffin Sections, 5 slides/pack |
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| TS-H5013 | IHC World | - | EUR 145 |
Kidney Tissue Slides, Normal Mouse Paraffin Sections, 5 slides/pack |
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| TS-M5203 | IHC World | - | EUR 145 |
Human Kidney Tissue Preparation Buffer 5: Normal Renal Mesangial Cells |
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| 9-80078 | CHI Scientific | 1 x 100 ml | Ask for price |
Mouse Kidney Tissue Preparation Buffer 6: Normal Renal Mesangial Cells |
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| 9-80175 | CHI Scientific | 1 x 100 ml | Ask for price |
Excessive phosphorus situation might inhibit gene expression. These outcomes indicated that SpPHT1;1 expression can be affected by exterior phosphorus focus. The outcomes of this examine are useful for additional analysis on the operate of phosphate transporter. It can also present theoretical foundation for additional improvement and utilization of S. polyrrhiza.
