The test allows for quality identification of IgG or IgM class antibodies directed against Borrelia burgdorferi bacteria (it covers detecting antibodies against genotypes: B. burgdorferi, B. afzellii, B. garinii, B. spielmanii), which causes the Lyme disease. The test should be used for confirming the results acquired for samples considered positive or uncertain in ELISA test.
In case of samples collected from patients with early stage of the infection, antibodies of IgM class dominate, while at later infection stages antibodies of IgG class would prevail. Positive test result in class IgG does not always indicate an active borreliosis. Antibodies of this class may prevail for years in human serum (plasma) or cerebrospinal fluid. It is possible to detect IgG antibodies coming from a past infection of Borrelia burgdorferi.
It should be remembered that a negative test result does not exclude the infection, especially at an early infection stage. It is possible that antibodies are not yet present in blood in the quantity facilitating their detection. Treatment with antibiotics at an early infection stage may stop development of antibodies, which would not then be detected in a Western blot test. When there is a suspicion of borreliosis, and test results are negative or uncertain, it is recommended to repeat the test after approximately three weeks.
Sample type: plasma, serum
Delivery and storage: at 4-8°C
Testing method: ELISA
Turn out time: up to 7 work days